Human Neural Stem Cells (H9-Derived) Kit

Catalog number: N7800200

Gibco™  Related applications: Stem Cell Culture

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Description

With GIBCO® Human Neural Stem Cells (H9-Derived), we allow neuroscience researchers who do not have access to human embryonic stem cells or human tissues the use of the hNSC by removing the complicated isolation or derivation process. The cells are derived from the NIH approved H9 (WA09) human embryonic stem cells (hESCs). Each vial of GIBCO® hNSCs contains>1 million viable cells that can be propagated as an adherent culture in complete StemPro® NSC SFM. GIBCO® hNSCs retain their normal female human karyotype and their potential to differentiate into neurons and glial cells after multiple passages. GIBCO® Human Neural Stem Cells (H9-Derived) Kit comes with 1 vial of cells, KnockOut™ DMEM⁄F-12 Basal Medium, StemPro® Neural Supplement, FGF Basic and EGF Recombinant proteins for optimal growth and expansion as adherent cells on matrix or suspended neurospheres. Unlike tissue derived cells, hESC derived NSCs offer more consistent performance and minimize supply concerns.
- Strong performance in adherent culture in a matrix and suspended neurosphere cultures.
- Upon proliferation in StemPro® NSC SFM, >80% of the cells retain their undifferentiated Phenotype. Internal experiments have shown >90% of the cells retain their undifferentiated state.
- Supports differentiation into Neurons, Astrocytes and Oligodendrocytes.
- Sold as stand-alone cells and as a kit. Kit comes with cells and required reagents.
- Minimal variability across lots and ready-to use system.
Neural stem cells (NSCs) are self-renewing, multipotent stem cells of nervous system that can differentiate into neurons, oliogdendrocytes, and astrocytes. Multipotent NSCs can be derived from embryonic stem cells or isolated from fetal or adult central nervous system. NSCs are an invaluable resource not only for neuroscience and stem cell studies such as regulation of neurogenesis, neurotransmitter and receptor functions, and stem cell differentiation, but also for tissue engineering, cell and genetic therapy, and transplantation experiments to treat neurodegenerative diseases and neurological disorders.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Form: Cryopreserved
Species: Human
Quantity: 1 kit
Cell Type: Stem Cells (Neural)
Product Size: 1 kit
Parental Cells: H9 Human ESC
Number of Cells: 1 x 106
Donor Attributes: Normal
Media Recommendation: StemPro® NSC SFM

Contents & storage

GIBCO® Human Neural Stem Cells (H9-Derived) Kit contains:
•1 vial of GIBCO® Human Neural Stem Cells (H9-Derived) (>1 × 10^6 viable cells⁄mL in freezing medium) and cryopreserved in liquid nitrogen (Store in liquid nitrogen)
•KnockOut™ DMEM?F-12 Basal Medium (Store in the dark 2 to 8°C)
•StemPro® Neural Supplement (Store frozen at -5 to -20°C)
•FGF Basic and EGF Recombinant proteins (Store at 2 to 8°C desiccated)

Documents

Manuals & protocols