MuLV Reverse Transcriptase

Catalog number: N8080018

Invitrogen™  Related applications: Reverse Transcription

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MuLV Reverse Transcriptase is a recombinant RNA-dependent DNA polymerase that uses single-stranded RNA as a template in the presence of a primer to synthesize a complementary DNA (cDNA) strand. It can be used for the preparation of cDNA libraries, for first-strand synthesis in RT-PCR, or in dideoxy sequencing reactions in place of Klenow enzyme, for G+C–rich regions. MuLV Reverse Transcriptase lacks endonuclease activity and has extremely low RNase H activity compared to AMV reverse transcriptase.
For Research Use Only. Not for use in diagnostic procedures.


Optimal Reaction Temperature: 42-52° C
Final Product Size(s): 5 kb or less
Reverse Transcriptase: MuLV
Ribonuclease H Activity: Reduced
Template: ssRNA
Sensitivity: High
Product Size: 5,000 units
Enzyme Function: RNA dependent DNA Polymerase
Shipping Condition: Dry Ice

Contents & storage

Contents: 100 µL of MuLV Reverse Transcriptase (50 U/µL). Store between -15 and -25°C


Manuals & protocols

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