Invitrogen™

NuPAGE™ Bis-Tris Welcome Pack, 4 to 12%, 10-well

Catalog number:  NP032A

Related applications:

Protein Electrophoresis & Western Blotting

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Description

The NuPAGE Bis-Tris Welcome Pack provides all of the necessary NuPAGE gels, buffers, and reagents you will need to begin using the Mini Gel Tank.

The NuPAGE Bis-Tris Welcome Pack contains:
Mini Gel Tank
• NuPAGE Bis-Tris mini gels (2 boxes, 20 gels)
NuPAGE MES SDS Running Buffer (20X)
NuPAGE LDS Sample Buffer (4X)
NuPAGE Sample Reducing Agent (10X)
PageRuler Plus Prestained Protein Ladder, 10 to 250 kDa

About the Mini Gel Tank
The Mini Gel Tank is compatible with all Invitrogen Novex, NuPAGE, and Bolt mini gels. Each Mini Gel Tank can accommodate up to two gels per run. The unique tank design enables convenient, side-by-side gel loading and enhanced viewing during use. Run times may vary depending on buffer conditions and power supplies used.

About NuPAGE Bis-Tris gels
Invitrogen NuPAGE Bis-Tris Protein gels are precast polyacrylamide gels designed to give optimal separation of a wide range of proteins under denaturing conditions. Unlike traditional Tris-glycine gels, NuPAGE Bis-Tris gels have a neutral pH environment that minimizes protein modifications. Use NuPAGE Bis-Tris gels for preparing proteins for sequencing, mass spectrometry, and any other techniques where protein integrity is crucial. Also, use NuPAGE gels for optimal results during day-to-day use.

Transfer
For transferring your proteins to a membrane, we recommend using for NuPAGE Transfer Buffer for traditional wet transfer using the Mini Blot Module. Alternatively, rapid semi-dry transfer can be done using the Invitrogen Power Blotter or rapid dry transfer using the iBlot 2 Gel Transfer Device.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Product Line: NuPAGE™
Shipping Condition: Approved for shipment at Room Temperature and wet ice
For Use With (Application): Protein Gel Electrophoresis
Kit Contents: Mini Gel Tank, NuPAGE Bis-Tris Mini Gels (2 Boxes, 20 Gels), NuPAGE Lds Sample Buffer (4X), NuPAGE Mes SDS Running Buffer (20X), NuPAGE Sample Reducing Agent (10X), PageRuler Plus Prestained Protein Ladder, 10 to 250 kd
Length (Metric): 8 cm
Length (Metric) Gel: 8 cm
Shelf Life: 12 Months
Storage Requirements: Up to 12 months at 4°C
Volume Per Well: 25 μL
Width (Metric): 8 cm
Width (Metric) Gel: 8 cm
Product Type: Bis-Tris Welcome Pack
Material: Bis-Tris Gel
Gel Size: Mini
Separation Range: 3.5 to 260 kd
Gel Percentage: 4 to 12%
Gel Type: Bis-Tris
Separation Type: Denaturing
Gel Thickness: 1.0 mm
For Use With (Equipment): Mini Gel Tank
Wells: 10-well
Quantity: Each

Contents & storage

• Mini Gel Tank
• NuPAGE 4-12% Bis-Tris Protein Gels, 1.0 mm, 10-well
• NuPAGE MES SDS Running Buffer (20X)
• NuPAGE LDS Sample Buffer (4X)
• NuPAGE Sample Reducing Agent (10X)
• PageRuler Plus Prestained Protein Ladder, 10 to 250 kDa