Invitrogen™

Bolt™ Bis-Tris Welcome Pack, 10%, 10-well

Catalog number:  NW0010A

Related applications:

Protein Electrophoresis & Western Blotting

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Description

The Bolt Welcome Pack provides all of the necessary Bolt gels and buffers you will need to begin using the Mini Gel Tank. The Welcome Pack contains:

Mini Gel Tank
• Bolt Bis-Tris Plus gels (2 boxes, 20 gels)
Bolt MES Running Buffer (20X)
Bolt LDS Sample Buffer (4X)
Bolt Sample Reducing Agent (10X)
PageRuler Plus Prestained Protein Ladder, 10 to 250 kDa

About the Mini Gel Tank
The Mini Gel Tank is compatible with Novex gels, NuPAGE gels, and Bolt Bis-Tris Plus gels. Each Mini Gel Tank can accommodate up to two gels per run. The unique tank design enables convenient side-by-side gel loading and enhanced viewing during use. Optimized conditions using constant voltage allow for Bolt Bis-Tris Plus gels to be run in approximately 35 minutes. Run times may vary depending on buffer conditions and power supplies used.

About Bolt Bis-Tris Plus Gels
Bolt Bis-Tris Plus gels are precast polyacrylamide gels designed for optimal separation of your small- to medium-sized proteins under denaturing conditions. Bolt Bis-Tris Plus gels are designed to deliver consistent gel performance and provide a neutral pH environment that minimizes protein modifications. Bolt gels are ideal for western blot transfer and analysis, and any other techniques where protein integrity is crucial. Also use Bolt gels to obtain optimal results for your day-to-day protein separation needs. Bolt Bis-Tris Plus gels come in four polyacrylamide concentrations: 8, 10, 12, and 4-12% gradient and multiple well formats. Bolt Bis-Tris gels feature wedge-shaped wells, which can easily load up to two times more sample volume allowing for detection of proteins in very dilute samples or visualization of low-abundance proteins.

For transferring your proteins to a membrane, we recommend using for Bolt Transfer Buffer for traditional wet transfer using the Mini Blot Module. Alternatively, rapid semi-dry transfer can be done using the Invitrogen Power Blotter or rapid dry transfer using the iBlot 2 Gel Transfer Device.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Product Line: Bolt™
Shipping Condition: Approved for shipment at Room Temperature and wet ice
For Use With (Application): Protein Gel Electrophoresis
Kit Contents: NuPAGE Lds Sample Buffer (4X), NuPAGE Mes SDS Running Buffer (20X), NuPAGE Sample Reducing Agent (10X), PageRuler Plus Prestained Protein Ladder, 10 to 250 kd, Mini Gel Tank, NuPAGE Bis-Tris Mini Gels (2 Boxes, 20 Gels)
Length (Metric): 8 cm
Length (Metric) Gel: 8 cm
Shelf Life: 12 Months
Storage Requirements: Up to 12 months at 4°C
Volume Per Well: 60 μL
Width (Metric): 8 cm
Width (Metric) Gel: 8 cm
Product Type: Bis-Tris Welcome Pack
Material: Bis-Tris Plus Gel
Gel Size: Mini
Separation Range: 3.5 to 260 kd
Gel Percentage: 10%
Gel Type: Bis-Tris
Separation Type: Denaturing
Gel Thickness: 1.0 mm
For Use With (Equipment): Mini Gel Tank
Wells: 10-well
Quantity: Each

Contents & storage

• Mini Gel Tank
• Bolt 10% Bis-Tris Plus Gels, 10-well
• Bolt MES Running Buffer (20X)
• Bolt LDS Sample Buffer (4X)
• Bolt Sample Reducing Agent (10X)
• PageRuler Plus Prestained Protein Ladder, 10 to 250 kDa