The new Mini Gel Tank includes cassette clamps with gray cam handles designed for use with the new 10 cm Bolt® mini gels as well as existing NuPAGE® and Novex® Tris-Glycine mini gels.
Invitrogen™

Bolt™ Welcome Pack A (4-12%, 10-well)

Catalog number:  NW0412A

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Description

The Bolt Welcome Pack provides all of the necessary Bolt gels and buffers you will need to begin using the Mini Gel Tank. The Welcome Pack A contains:

Mini Gel Tank
Bolt 4-12% Bis-Tris Plus gels, 10-well (2 boxes)
Bolt MES Running Buffer (20X)
Bolt LDS Sample Buffer (4X)
Bolt Sample Reducing Agent (10X)
SeeBlue Plus2 Pre-Stained Standard

About the Mini Gel Tank
The Mini Gel Tank is compatible with Novex gels, NuPAGE gels, and Bolt Bis-Tris Plus gels. Each Mini Gel Tank can accommodate up to two gels per run. The unique tank design enables convenient side-by-side gel loading and enhanced viewing during use. Optimized conditions using constant voltage allow for Bolt Bis-Tris Plus gels to be run in approximately 35 minutes. Run times may vary depending on buffer conditions and power supplies used.

About Bolt Bis-Tris Plus Gels
Bolt Bis-Tris Plus gels are precast polyacrylamide gels designed for optimal separation of your small- to medium-sized proteins under denaturing conditions. Bolt Bis-Tris Plus gels are designed to deliver consistent gel performance and provide a neutral pH environment that minimizes protein modifications. Bolt gels are ideal for western blot transfer and analysis, and any other techniques where protein integrity is crucial. Also use Bolt gels to obtain optimal results for your day-to-day protein separation needs. Bolt Bis-Tris Plus gels come in four polyacrylamide concentrations: 8, 10, 12, and 4-12% gradient and multiple well formats. Bolt Bis-Tris gels feature wedge-shaped wells, which can easily load up to two times more sample volume allowing for detection of proteins in very dilute samples or visualization of low-abundance proteins.

For transferring your proteins to a membrane, we recommend using for Bolt Transfer Buffer for traditional wet transfer using the Mini Blot Module. Alternatively, rapid semi-dry transfer can be done using the Invitrogen Power Blotter or rapid dry transfer using the iBlot 2 Gel Transfer Device.

WARNING: Cancer – www.P65Warnings.ca.gov
For Research Use Only. Not for use in diagnostic procedures.

Specifications

For Use With (Equipment): Bolt®
Capacity: Up to 2 gels per run
Gel Compatibility: Novex® Mini Gels
Product Line: Bolt™
Product Size: 1 kit
Shipping Condition: Approved for shipment at Room Temperature or on Wet Ice

Contents & storage

Contains:
• Mini Gel Tank
• 2 boxes of Bolt™ 4-12% Bis-Tris Plus gels, 10-well (20 gels)
• 500 mL of Bolt™ MES SDS Running Buffer (20X)
• 10 mL of Bolt™ LDS Sample Buffer (4X)
• 10 mL of Bolt™ Sample Reducing Agent (10X)
• 500 µL SeeBlue™ Plus2 Pre-Stained Standard

Documents

Manuals & protocols