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Oregon Green® 488 Carboxylic Acid Diacetate (carboxy-DFFDA) - 6-Isomer

Catalog number: O6151

Molecular Probes™  Related applications: Cell Tracing & Tracking | Cell Viability, Proliferation & Function | Cellular Imaging | Target-Based ADME⁄Tox Assays

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Description

Oregon Green® 488 carboxylic acid diacetate (carboxy-DFFDA) is a cell-permeant esterase substrate that can serve as a viability probe that measures both enzymatic activity, which is require to activate its fluorescence, and cell-membrane integrity, which is required for intracellular retention of their fluorescent product. Upon hydrolysis by intracellular esterases, this AM ester yields Oregon Green® 488 (excitation/emission maxima ~496/524 nm). This fluorinated analog of fluorescein overcomes some of the key limitations of fluorescein, including greater photostability and a lower pKa (pKa ~ 4.7 versus 6.4 for fluorescein), making its fluorescence essentially pH insensitive in the physiological pH range.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Reagent Type: carboxy-DFFDA & Related Compounds
Product Size: 5 mg
Shipping Condition: Room Temperature

Contents & storage

Store in freezer (-5 to -30°C).

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