The M-phase-promoting factor modulates the sensitivity of the Ca2+ stores to inositol 1,4,5-trisphosphate via the actin cytoskeleton.
AuthorsLim D, Ercolano E, Kyozuka K, Nusco GA, Moccia F, Lange K, Santella L
JournalJ Biol Chem
PubMed ID12867432
'The resumption of the meiotic cycle (maturation) induced by 1-methyladenine in prophase-arrested starfish oocytes is indicated by the breakdown of the germinal vesicle and is characterized by the increased sensitivity of the Ca2+ stores to inositol 1,4,5-trisphosphate (InsP3) to InsP3 starting at the animal hemisphere (where the germinal vesicle was ... More
NAADP: a new second messenger for glucose-induced Ca2+ responses in clonal pancreatic beta cells.
AuthorsMasgrau R, Churchill GC, Morgan AJ, Ashcroft SJ, Galione A
JournalCurr Biol
PubMed ID12573222
'Important questions remain concerning how elevated blood glucose levels are coupled to insulin secretion from pancreatic beta cells and how this process is impaired in type 2 diabetes. Glucose uptake and metabolism in beta cells cause the intracellular Ca(2+) concentration ([Ca(2+)](i)) to increase to a degree necessary and sufficient for ... More
Nicotine induces calcium spikes in single nerve terminal varicosities: a role for intracellular calcium stores.
AuthorsBrain KL, Trout SJ, Jackson VM, Dass N, Cunnane TC
JournalNeuroscience
PubMed ID11566509
'While nicotine is known to act at neuronal nicotinic acetylcholine receptors (nAChRs) to facilitate neurotransmitter release, the mechanisms underlying this action are poorly understood. Some of its effects are known to be mediated by presynaptic receptors. In the mouse vas deferens nicotine (10-30 microM) transiently increased the force of neurogenic ... More
Prolonged inactivation of nicotinic acid adenine dinucleotide phosphate-induced Ca2+ release mediates a spatiotemporal Ca2+ memory.
AuthorsChurchill GC, Galione A
JournalJ Biol Chem
PubMed ID11139579
'Although numerous extracellular stimuli are coupled to increases in intracellular Ca(2+), different stimuli are thought to achieve specificity by eliciting different spatiotemporal Ca(2+) increases. We investigated the effect of nicotinic acid adenine dinucleotide phosphate (NAADP) inactivation on spatiotemporal Ca(2+) signals in intact sea urchin eggs. The photorelease of NAADP but ... More
Transmitter-evoked local calcium release stabilizes developing dendrites.
AuthorsLohmann C, Myhr KL, Wong RO
JournalNature
PubMed ID12110889
In the central nervous system, dendritic arborizations of neurons undergo dynamic structural remodelling during development. Processes are elaborated, maintained or eliminated to attain the adult pattern of synaptic connections. Although neuronal activity influences this remodelling, it is not known how activity exerts its effects. Here we show that neurotransmission-evoked calcium ... More
Imaging calcium dynamics in the nervous system by means of ballistic delivery of indicators.
AuthorsKettunen P, Demas J, Lohmann C, Kasthuri N, Gong Y, Wong RO, Gan WB
JournalJ Neurosci Methods
PubMed ID12234633
The use of fluorescence-based calcium indicators has, over the years, unraveled important calcium-dependent mechanisms underlying neuronal function and development. However, difficulties associated with the loading of calcium indicators have limited their widespread use, particularly for the study of neuronal processing in the adult nervous system. Here, we show that in ... More
Spatial control of Ca2+ signaling by nicotinic acid adenine dinucleotide phosphate diffusion and gradients.
AuthorsChurchill GC, Galione A
JournalJ Biol Chem
PubMed ID11006280
Intracellular Ca(2+) is able to control numerous cellular responses through complex spatiotemporal organization. Ca(2+) waves mediated by inositol trisphosphate or ryanodine receptors propagate by Ca(2+)-induced Ca(2+) release and therefore do not have an absolute requirement for a gradient in either inositol trisphosphate or cyclic ADP-ribose, respectively. In contrast, we report ... More
Calcium signalling in the guidance of nerve growth by netrin-1.
AuthorsHong K, Nishiyama M, Henley J, Tessier-Lavigne M, Poo M
JournalNature
PubMed ID10638760
Pathfinding by growing axons in the developing nervous system is guided by diffusible or bound factors that attract or repel the axonal growth cone. The cytoplasmic signalling mechanisms that trigger the responses of the growth cone to guidance factors are mostly unknown. Previous studies have shown that the level and ... More