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Oregon Green® 514 Phalloidin

Catalog number: O7465

Molecular Probes™  Related applications: Cell Structure | Cell Tracing & Tracking

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Oregon Green® 514 phalloidin is a high-affinity F-actin probe conjugated to green fluorescent Oregon Green® 514 dye.

Selectively stains F-actin
Outstanding fluorescence performance
Excitation/Emission: 511/528 nm
Superior to antibody staining
Optimal for fixed and permeabilized samples
pH insensitive under physiological conditions

Get Superior Results in Actin Staining Studies
Phalloidin is a bicyclic peptide belonging to a family of toxins isolated from the deadly Amanita phalloides "death cap" mushroom and is commonly used in imaging applications to selectively label F-actin. Fluorescently-labeled phalloidin has virtually identical binding properties with actin from different species including plants and animals. Phalloidin binds F-actin with high selectivity while Oregon Green® 514 provides green fluorescence with brightness superior to fluorescein. Oregon Green® 514 is a fluorinated analog of fluorescein with greater photostability and a lower pKa, making its fluorescence essentially pH insensitive in the physiological pH range. Demonstrating very little nonspecific staining, Oregon Green® 514 phalloidin allows high-contrast discrimination of actin staining.

Use in Multiple Applications
Oregon Green® 514 phalloidin can be used to visualize and quantitate F-actin in tissue sections, cell cultures, or cell-free preparations. Oregon Green® 514 phalloidin staining is fully compatible with other fluorescent stains used in cellular analyses including fluorescent proteins, Qdot® nanocrystals, and Alexa Fluor® conjugates including secondary antibodies.

For research use only. Not intended for human or animal therapeutic or diagnostic use.

Related Links
Read about labeling F-Actin with phallotoxins.
Create a Cellular Masterpiece with Alexa Fluor® Phalloidin using the Cell Paint Tool.
For Research Use Only. Not for use in diagnostic procedures.


Label or Dye: Oregon Green® 514
Sub-Cellular Localization: Cytoskeleton, Actin
Product Size: 300 units
Excitation⁄Emission (nm): 511⁄528
Shipping Condition: Room Temperature

Contents & storage

Store in freezer (-5 to -30°C) and protect from light.


Manuals & protocols

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