The crystal structure of (S)-3-O-geranylgeranylglyceryl phosphate synthase reveals an ancient fold for an ancient enzyme.
AuthorsPayandeh J, Fujihashi M, Gillon W, Pai EF
JournalJ Biol Chem
PubMed ID16377641
'We report crystal structures of the citrate and sn-glycerol-1-phosphate (G1P) complexes of (S)-3-O-geranylgeranylglyceryl phosphate synthase from Archaeoglobus fulgidus (AfGGGPS) at 1.55 and 2.0 A resolution, respectively. AfGGGPS is an enzyme that performs the committed step in archaeal lipid biosynthesis, and it presents the first triose phosphate isomerase (TIM)-barrel structure with ... More
Thermotoga maritima MazG protein has both nucleoside triphosphate pyrophosphohydrolase and pyrophosphatase activities.
AuthorsZhang J, Zhang Y, Inouye M,
JournalJ Biol Chem
PubMed ID12657645
'MazG proteins form a widely conserved family among bacteria, but their cellular function is still unknown. Here we report that Thermotoga maritima MazG protein (Tm-MazG), the product of the TM0913 gene, has both nucleoside triphosphate pyrophosphohydrolase (NTPase) and pyrophosphatase activities. Tm-MazG catalyzes the hydrolysis of all eight canonical ribo- and ... More
Binding modes of zaragozic acid A to human squalene synthase and staphylococcal dehydrosqualene synthase.
AuthorsLiu CI, Jeng WY, Chang WJ, Ko TP, Wang AH,
JournalJ Biol Chem
PubMed ID22474324
'Zaragozic acids (ZAs) belong to a family of fungal metabolites with nanomolar inhibitory activity toward squalene synthase (SQS). The enzyme catalyzes the committed step of sterol synthesis and has attracted attention as a potential target for antilipogenic and antiinfective therapies. Here, we have determined the structure of ZA-A complexed with ... More
Selectivity of fungal sesquiterpene synthases: role of the active site's H-1 alpha loop in catalysis.
'Sesquiterpene synthases are responsible for the cyclization of farnesyl pyrophosphate into a myriad of structurally diverse compounds with various biological activities. We examine here the role of the conserved active site H-a1 loop in catalysis in three previously characterized fungal sesquiterpene synthases. The H-a1 loops of Cop3, Cop4, and Cop6 ... More
Mycobacterial MazG is a novel NTP pyrophosphohydrolase involved in oxidative stress response.
AuthorsLu LD, Sun Q, Fan XY, Zhong Y, Yao YF, Zhao GP,
JournalJ Biol Chem
PubMed ID20529853
MazG nucleoside triphosphate pyrophosphohydrolase (NTP-PPase, EC 3.6.1.8) from the avirulent Mycobacterium tuberculosis H37Ra contains a spontaneous mutation on a highly conserved residue, resulting in an A219E substitution (MtMazG[A219E]). In this work, we show that mycobacterial MazG from either the virulent M. tuberculosis H37Rv (MtMazG) or the fast-growing Mycobacterium smegmatis (MsMazG) ... More
Identification of the inorganic pyrophosphate metabolizing, ATP substituting pathway in mammalian spermatozoa.