PeppermintStick™ Phosphoprotein Molecular Weight Standards

PeppermintStick™ Phosphoprotein Molecular Weight Standards - FAQs

View additional product information for PeppermintStick™ Phosphoprotein Molecular Weight Standards - FAQs (P33305)

5 product FAQs found

Do protein standards run differently on a Zymogram gel compared to a regular Tris-Glycine gel?

Zymogram gels are essentially Tris-Glycine gels containing the substrate. Protein standards run based solely on the percentage of acrylamide and hence should run the same in both kinds of gels. It is quite possible though that if the standard is prestained, the proteins will appear a different color because of the staining (or pre-staining) of the Zymogram gels.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Can I use any of your protein standards for estimation of protein quantity (protein quantitation)?

Our protein standards are not designed for protein quantitation.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

What is the recommended gel loading volume for your protein standards?

Please find this information in the respective manuals for the individual protein standards.

Find additional tips, troubleshooting help, and resources within our Protein Standards and Ladders Support Center.

Do I need to boil your protein standards before loading on the gel?

Our protein standards are ready to load. We do not recommend heating them as this may cause protein degradation.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Do I have to add reducing agent to your protein standards?

Except for our NativeMark Unstained Protein Standard (designed for native electrophoresis), all of the other unstained and prestained standards we offer (Invitrogen Sharp, SeeBlue, SeeBlue Plus2, BenchMark, HiMark) have been pre-reduced (by a proprietary method). Hence, you do not need to add reducing agent.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.