pHrodo™ Deep Red TFP Ester, 3 x 100 μg

pHrodo™ Deep Red TFP Ester, 3 x 100 μg - FAQs

View additional product information for pHrodo™ Dyes for Amine Labeling - FAQs (P36013, P36012, P36011, P36010, P35359, P35358)

4 product FAQs found

May I store pHrodo Deep Red TFP Ester (Cat. Nos. P35358, P35259) labeled antibodies or other proteins in solutions containing sodium azide?

We do not recommend storing antibodies or proteins labeled with pHrodo Deep Red TFP Ester (Cat. Nos. P35358, P35359) in solutions containing sodium azide, as pHrodo Deep Red is not stable in the presence of sodium azide. Consider using an alternative, non-azide-based anti-microbial.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

After labeling with pHrodo Deep Red TFP Ester (Cat. Nos. P35358, P35259), may I store the dye-protein conjugated in other buffers?

Yes, after labeling with pHrodo Deep Red TFP Ester (Cat. Nos. P35358, P35259) you can store your dye-conjugated protein in other buffers based on what is ideal for maintaining the stability of your protein. We do not recommend storing pHrodo Deep Red-labeled proteins in solutions containing sodium azide.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

After labeling with pHrodo Deep Red TFP Ester (Cat. Nos. P35358, P35259), do I have to store my protein at 2–8 degrees C or can I store it at other temperatures?

Yes, after labeling with pHrodo Deep Red TFP Ester (Cat. Nos. P35358, P35259) you can store your dye-conjugated protein at other temperatures based on what is ideal for maintaining the stability of your protein.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Does my protein have to be at an exact concentration of 2.2 mg/mL as noted in the labeling protocol for pHrodo Deep Red TFP Ester (Cat. Nos. P35358, P35359)?

The final protein concentration of ~2 mg/mL for the labeling reaction of pHrodo Deep Red TFP Ester (Cat. Nos. P35358, P35359) affects good reaction kinetics and good labeling with the recommended range of the dye-to-protein molar ratio (MR) from 5-20 moles of dye per mole of protein. You may use a protein concentration >2 mg/mL assuming the solution does not become more viscous, or the protein becomes more aggregated and, optimize the MR.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.