Invitrogen™

pHrodo™ Red Epidermal Growth Factor (EGF) Conjugate

Catalog number: P35374
Invitrogen™

pHrodo™ Red Epidermal Growth Factor (EGF) Conjugate

Catalog number: P35374
Catalog Number
P35374
Unit Size
20 µg
Price (USD)
Price: 480.00
Your Price:
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
P3537420 µg
Price: 480.00
Your Price:
-
Product Overview
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pHrodo Red Epidermal Growth Factor (EGF) Conjugate is used for the detection of internalized EGF receptor in cells. Fluorescently labeled EGF has enabled scientists to investigate receptor-membrane interactions, study receptor distribution, calculate rate constants for the interaction of EGF with its receptor, and more. Fluorescent labels that respond to a pH change add another level of detection. pHrodo Red is weakly fluorescent outside of cells at neutral pH, but when internalized specifically through EGF endocytosis, it becomes brightly fluorescent in the acidic endosomes. This allows the measurement of internalized EGF, as well as the dynamic monitoring of EGF internalization in live cells.

Features of this EGF conjugate include:

• pHrodo Red dye conjugated directly to highly active human recombinant EGF molecule
• Excitation/emission of 560/585 nm
• Excellent photostability
• Appropriate for multiplexing with green fluorophores such as GFP, FITC, and Alexa Fluor 488

pHrodo Red Epidermal Growth Factor (EGF) Conjugate is compatible with various platforms, such as traditional fluorescence microscopy, high content screening (HCS), flow cytometery, and microplate-based fluorimetry or high throughput screening (HTS). This reagent is also compatible with various benchtop instruments such as the Evos, FLoid, Tali, and Attune instruments.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Format
Lyophilized
Quantity
20 µg
Conjugate
pHrodo Red
Detection Method
Fluorescent
Excitation/Emission
560⁄585
For Use With (Equipment)
Fluorescence Microscope, Flow Cytometer, Fluorometer, High Content Instrument
Label or Dye
pH-Sensitive Dyes
Label Type
pH-Sensitive Dyes
Sub Cellular Localization
Endosomes, Lysosomes
Protein Family
Growth Factors & Receptors
Form
Lyophilized Powder
Protein Subtype
EGF (Epidermal Growth Factor)
Color
Orange
Product Line
pHrodo™

Contents & Storage

Store at -5 to -30°C, dessicated and protected from light.