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pHrodo™ Red Epidermal Growth Factor (EGF) Conjugate

Catalog number:  P35374

 Related applications:

Cell Viability, Proliferation & Function

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pHrodo Red Epidermal Growth Factor (EGF) Conjugate is used for the detection of internalized EGF receptor in cells. Fluorescently labeled EGF has enabled scientists to investigate receptor-membrane interactions, study receptor distribution, calculate rate constants for the interaction of EGF with its receptor, and more. Fluorescent labels that respond to a pH change add another level of detection. pHrodo Red is weakly fluorescent outside of cells at neutral pH, but when internalized specifically through EGF endocytosis, it becomes brightly fluorescent in the acidic endosomes. This allows the measurement of internalized EGF, as well as the dynamic monitoring of EGF internalization in live cells.

Features of this EGF conjugate include:

• pHrodo Red dye conjugated directly to highly active human recombinant EGF molecule
• Excitation/emission of 560/585 nm
• Excellent photostability
• Appropriate for multiplexing with green fluorophores such as GFP, FITC, and Alexa Fluor 488

pHrodo Red Epidermal Growth Factor (EGF) Conjugate is compatible with various platforms, such as traditional fluorescence microscopy, high content screening (HCS), flow cytometery, and microplate-based fluorimetry or high throughput screening (HTS). This reagent is also compatible with various benchtop instruments such as the Evos, FLoid, Tali, and Attune instruments.
For Research Use Only. Not for use in diagnostic procedures.


Color: Orange
Detection Method: Fluorescent
Emission Class: Visible
Excitation Class: Visible
Excitation⁄Emission (nm): 560⁄585
For Use With (Equipment): Flow Cytometer, Fluorescence Microscope, Fluorometer, High Content Instrument
Form: Lyophilized
Label or Dye: pH-Sensitive Dyes
Product Line: pHrodo™
Product Size: 20 µg
Sub-Cellular Localization: Endosomes, Lysosomes

Contents & storage

Store at -5 to -30°C, dessicated and protected from light.


Manuals & protocols