Invitrogen™

pHrodo™ Red and Green Epidermal Growth Factor (EGF) and pHrodo™ Red Transferrin Conjugates

Catalog number: P35375
Invitrogen™

pHrodo™ Red and Green Epidermal Growth Factor (EGF) and pHrodo™ Red Transferrin Conjugates

Catalog number: P35375
Detect internalized EGF or transferrin with highly active, pH-sensitive pHrodo Red and pHrodo Green Epidermal Growth Factor (EGF) conjugates, and the pHrodo Red Transferrin Conjugate. These conjugates consist of pHrodo dyes directly linked to highly active EGF or transferrin molecules, which can be used in applications including HCS, flow cytometry, microplate fluorometry, and HTS to measure internalized EGF and transferrin molecules. The pHrodo Red and pHrodo Green dyes fluoresce brightly when exposed to acidic environments, such as those found in endosomes and lysosomes.
 
Catalog Number
P35375
Unit Size
20 µg
Description
pHrodo™ Green Epidermal Growth Factor (EGF) Conjugate
Price (USD)
Price: 528.00
Your price:
Availability
-
Full specifications
Quantity
ColorGreen
DescriptionpHrodo™ Green Epidermal Growth Factor (EGF) Conjugate
Detection MethodFluorescence
For Use With (Application)Cell Analysis
For Use With (Equipment)Fluorescence Microscope, Flow Cytometer, Fluorometer, High Content Analysis Instrument
Product TypeCell Labeling Solution
FormLyophilized Powder
FormatLyophilized
Product LinepHrodo™
Quantity20 μg
Sub Cellular LocalizationEndosomes, Lysosomes
Excitation/Emission509/533
Protein FamilyGrowth Factors and Receptors
Unit Size20 µg
Catalog Number
Unit Size
1 mg
Description
pHrodo™ Red Transferrin Conjugate
Price (USD)
Price: 590.00
Online price: 516.65
Your price:
Availability
-
Full specifications
Quantity
ColorOrange
DescriptionpHrodo™ Red Transferrin Conjugate
Detection MethodFluorescence
For Use With (Application)Cell Analysis
For Use With (Equipment)Fluorescence Microscope, Flow Cytometer, Fluorometer, High Content Analysis Instrument
Product TypeCell Labeling Solution
Dye TypepHrodo Red
FormLyophilized Powder
FormatLyophilized
Product LinepHrodo™
Quantity1 mg
Sub Cellular LocalizationEndosomes
Excitation/Emission560/585
Unit Size1 mg
Catalog Number
Unit Size
20 µg
Description
pHrodo™ Red Epidermal Growth Factor (EGF) Conjugate
Price (USD)
Price: 530.00
Online price: 505.65
Your price:
Availability
-
Full specifications
Quantity
ColorOrange
DescriptionpHrodo™ Red Epidermal Growth Factor (EGF) Conjugate
Detection MethodFluorescence
For Use With (Application)Cell Analysis
For Use With (Equipment)Fluorescence Microscope, Flow Cytometer, Fluorometer, High Content Analysis Instrument
Product TypeCell Labeling Solution
FormLyophilized Powder
FormatLyophilized
Product LinepHrodo™
Quantity20 μg
Sub Cellular LocalizationEndosomes, Lysosomes
Excitation/Emission560/585
Protein FamilyGrowth Factors and Receptors
Unit Size20 µg
Showing 3 of 3
Catalog NumberSpecificationsUnit SizeDescriptionPrice (USD)AvailabilityQuantity
P35375Full specifications
20 µgpHrodo™ Green Epidermal Growth Factor (EGF) Conjugate
Price: 528.00
Your price:
-
ColorGreen
DescriptionpHrodo™ Green Epidermal Growth Factor (EGF) Conjugate
Detection MethodFluorescence
For Use With (Application)Cell Analysis
For Use With (Equipment)Fluorescence Microscope, Flow Cytometer, Fluorometer, High Content Analysis Instrument
Product TypeCell Labeling Solution
FormLyophilized Powder
FormatLyophilized
Product LinepHrodo™
Quantity20 μg
Sub Cellular LocalizationEndosomes, Lysosomes
Excitation/Emission509/533
Protein FamilyGrowth Factors and Receptors
Unit Size20 µg
P35376Full specifications
1 mgpHrodo™ Red Transferrin Conjugate
Price: 590.00
Online price: 516.65
Your price:
-
ColorOrange
DescriptionpHrodo™ Red Transferrin Conjugate
Detection MethodFluorescence
For Use With (Application)Cell Analysis
For Use With (Equipment)Fluorescence Microscope, Flow Cytometer, Fluorometer, High Content Analysis Instrument
Product TypeCell Labeling Solution
Dye TypepHrodo Red
FormLyophilized Powder
FormatLyophilized
Product LinepHrodo™
Quantity1 mg
Sub Cellular LocalizationEndosomes
Excitation/Emission560/585
Unit Size1 mg
P35374Full specifications
20 µgpHrodo™ Red Epidermal Growth Factor (EGF) Conjugate
Price: 530.00
Online price: 505.65
Your price:
-
ColorOrange
DescriptionpHrodo™ Red Epidermal Growth Factor (EGF) Conjugate
Detection MethodFluorescence
For Use With (Application)Cell Analysis
For Use With (Equipment)Fluorescence Microscope, Flow Cytometer, Fluorometer, High Content Analysis Instrument
Product TypeCell Labeling Solution
FormLyophilized Powder
FormatLyophilized
Product LinepHrodo™
Quantity20 μg
Sub Cellular LocalizationEndosomes, Lysosomes
Excitation/Emission560/585
Protein FamilyGrowth Factors and Receptors
Unit Size20 µg
Showing 3 of 3
The pHrodo Red and pHrodo Green Epidermal Growth Factor (EGF) conjugates consist of pHrodo Red or pHrodo Green dye directly conjugated to a highly active human recombinant EGF molecule and are used for the detection of internalized EGF receptor in cells. Fluorescently labeled EGF has enabled scientists to investigate receptor-membrane interactions, study receptor distribution, calculate rate constants for the interaction of EGF with its receptor, and more. Fluorescent labels that respond to a pH change add another level of detection.

pHrodo Red Transferrin Conjugate consists of pHrodo Red dye directly conjugated to a highly active transferrin molecule and is used for the detection of internalized transferrin in cells. Transferrin is a monomeric serum glycoprotein (∼80,000 daltons) that binds to a specific receptor on the surface of vertebrate cells and delivers up to two Fe3+ atoms via receptor-mediated endocytosis. Once iron-carrying transferrin proteins are inside endosomes, the acidic environment favors dissociation of iron from the transferrin–receptor complex. Following the release of iron, the apotransferrin is recycled to the plasma membrane, where it is released from its receptor to scavenge more iron.

The highly photostable pHrodo Red and pHrodo Green dyes are weakly fluorescent outside of cells at neutral pH, but when internalized specifically through EGF or transferrin endocytosis, become brightly fluorescent in acidic endosomes. This allows the measurement of internalized EGF or transferrin, as well as the dynamic monitoring of EGF or transferrin internalization in live cells.

The pHrodo Red EGF Conjugate and the pHrodo Red Transferrin Conjugate have excitation/emission spectra of 560/585 nm and can be multiplexed with a wide variety of blue, red, and far-red dyes reporters such as Mitosox Red, CellEvent Caspase 3/7 Red, NucBlue, RFPs, and Mitotracker Deep Red, among many others. The pHrodo Green EGF Conjugate has excitation/emission spectra of 509/533 nm and can be multiplexed with a wide variety of blue, red, and far-red dyes reporters such as Mitosox Red, CellEvent Caspase 3/7 Red, NucBlue, RFPs, and Mitotracker Deep Red, among many others.

The pHrodo Red and pHrodo Green EGF conjugates and the pHrodo Red Transferrin Conjugate are compatible in applications including traditional fluorescence microscopy, high content screening (HCS), flow cytometry, and microplate-based fluorimetry or high throughput screening (HTS). The conjugates are also compatible with various widefield or confocal fluorescence microscopes, flow cytometers, fluorescence plate readers, and high content instruments.

For Research Use Only. Not for use in diagnostic procedures.

Contents & Storage

Store at –5° to –30°C, desiccate, and protect from light.

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