Additional Autophagy Indicators—Now able to Multiplex!
Moving Autophagy research to the next level; announcing the launch of the Premo™ Autophagy Tandem Sensor!
Use ProLong® Live Antifade Reagent for brightest fluorescent images with live cells

Premo™ Autophagy Sensor LC3B-GFP (BacMam 2.0)

Catalog number: P36235

Molecular Probes™  Related applications: Cell Viability, Proliferation & Function

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Description

Premo™ Autophagy Sensor combines the selectivity of a LC3B-green fluorescent protein (GFP) chimera with the transduction efficiency of BacMam technology, enabling unambiguous visualization of this protein. BacMam reagents (insect Baculovirus with a Mammalian promoter) are non-replicating in mammalian cells and thus safe to handle. They are also non-cytotoxic and ready-to-use. Unlike expression vectors, BacMam reagents enable titratable and reproducible expression and offer high co-transduction efficiency, therefore multiple BacMam reagents can readily be used in the same cell. Recent improvements made to the BacMam system enable efficient transduction in a wider variety of cells including neurons and neural stem cells (NSCs) with an easy, one-step protocol. Now, to visualize autophagy, simply add the BacMam LC3B-GFP to the cells and incubate overnight for protein expression. Each Premo™ Autophagy Sensor Kit includes the BacMam LC3B-FP, a control BacMam LC3B (G120A)-FP (mutation on the control BacMam LC3B prevents cleavage and subsequent lipidation during normal autophagy, thus protein localization should remain cytosolic and diffuse) and chloroquine diphosphate to artificially induce autophagosome accumulation.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Color: Green
Format: Slide(s)
Label or Dye: GFP (EmGFP)
Product Size: 1 kit
Emission Class: Visible
Detection Method: Fluorescent
Excitation Class: Visible
For Use With (Equipment): Fluorescence Microscope, Confocal Microscope, High Content Instrument
Excitation⁄Emission (nm): 485⁄520
Shipping Condition: Wet Ice

Contents & storage

Autophagy Sensor Kit includes the BacMam LC3B-FP, a control BacMam LC3B (G120A)-RFP and chloroquine diphosphate.
Store at 2-6°C, protected from light. Do Not Freeze.

Documents

Manuals & protocols