ProLong™ Gold Antifade Mountant

HeLa cells were plated on coverslips overnight. The next day the cells were fixed and permeabilized using the Image-iT® Fixation/Permeabilization Kit (R37602) according to protocol. Cells were the

BPAE cells were plated on coverslips overnight.  The next day the cells were fixed and permeabilized using the Image-iT® Fixation/Permeabilization Kit (R37602) according to protocol.  Cells were t

HeLa cells were plated on coverslips overnight.  The next day cells were fixed and permeabilized using the Image-iT® Fixation/Permeabilization Kit (R37602) according to protocol.  Cells were then

HeLa cells were plated on coverslips overnight.  The next day cells were fixed and permeabilized using the Image-iT® Fixation/Permeabilization Kit (R37602) according to protocol.  Cells were then

BPAE cells were plated on coverslips overnight.  The next day the cells were fixed and permeabilized using the Image-iT® Fixation/Permeabilization Kit (R37602) according to protocol.  Cells were t

HeLa cells were plated on coverslips overnight.  The next day the cells were fixed and permeabilized using the Image-iT® Fixation/Permeabilization Kit (R37602) according to protocol.  Cells were t

BPAE cells were plated on coverslips overnight.  The next day the cells were fixed and permeabilized using the Image-iT® Fixation/Permeabilization Kit (R37602) according to protocol.  Cells were t

HeLa cells were plated on coverslips overnight. The next day the cells were fixed and permeabilized using the Image-iT® Fixation/Permeabilization Kit (R37602) according to protocol. Cells were the

HeLa cells were plated on coverslips overnight.  The next day the cells were fixed and permeabilized using the Image-iT® Fixation/Permeabilization Kit (R37602) according to protocol.  Cells were t

HDFn cells were fixed, permeabilized, and blocked using the Image-iT® Fixation/Permeabilization Kit (R37602). Peroxisomes were labeled with an anti-PMP70 antibody (718300) followed by detection with a

CAKI cells were plated on coverslips overnight.  The next day cells were fixed and permeabilized using the Image-iT® Fixation/Permeabilization Kit (R37602) according to protocol. Following suppres

HeLa cell transfected with pShooter pCMV/myc/mito/GFP, then fixed and permeabilized. Green-fluorescent protein (GFP) localized in the mitochondria was labeled with anti-GFP mouse IgG_2a (Cat

CAKI cells were plated on coverslips overnight.  The next day cells were fixed and permeabilized using the Image-iT® Fixation/Permeabilization Kit (R37602) according to protocol. Following suppres

HeLa cells were plated on coverslips overnight.  The next day cells were fixed and permeabilized using the Image-iT® Fixation/Permeabilization Kit (R37602) according to protocol.  Cells were then

HeLa cells were plated on coverslips overnight.  The next day cells were fixed and permeabilized using the Image-iT® Fixation/Permeabilization Kit (R37602) according to protocol.  Cells were then

HDFn cells were fixed, permeabilized, and blocked using the Image-iT® Fixation/Permeabilization Kit (R37602). Golgi were labeled with an anti-golgin 97 antibody (A21270) followed by detection with an

HeLa cells were plated on coverslips overnight. The next day the cells were fixed and permeabilized using the Image-iT® Fixation/Permeabilization Kit (R37602) according to protocol. Cells were the

HeLa cells were plated on coverslips overnight.  The next day cells were fixed and permeabilized using the Image-iT® Fixation/Permeabilization Kit (R37602) according to protocol.  Cells were then

CAKI cells were plated on coverslips overnigh. The next day cells were fixed and permeabilized using the Image-iT® Fixation/Permeabilization Kit (R37602) according to protocol. Peroxisomes were la

A 20-second time series showing enhanced resistance to photobleaching afforded by ProLong® Gold antifade reagent. Fixed bovine pulmonary artery endothelial cells were labeled with anti–α-t

BPAE cells were plated on coverslips overnight.  The next day cells were fixed and permeabilized using the Image-iT® Fixation/Permeabilization Kit (R37602) according to protocol.  Cells were then

BPAE cells were plated on coverslips overnight.  The next day cells were fixed and permeabilized using the Image-iT® Fixation/Permeabilization Kit (R37602) according to protocol and stained with N

HeLa cells were plated on coverslips overnight.  The next day cells were fixed and permeabilized using the Image-iT® Fixation/Permeabilization Kit (R37602) according to protocol.  Cells were then

HeLa cells were plated on coverslips overnight. The next day the cells were fixed and permeabilized using the Image-iT® Fixation/Permeabilization Kit (R37602) according to protocol. Cells were the

BPAE cells were plated on coverslips overnight.  The next day the cells were fixed and permeabilized using the Image-iT® Fixation/Permeabilization Kit (R37602) according to protocol.  Cells were t

Metabolic labeling of cell-surface glycoproteins. Jurkat cells were cultured for 24 hours in the presence of 25 µM ManLev tetraacetate (Cat. no. L20493). Following treatment, the cell surface–expres

HeLa cells were plated on coverslips overnight.  The next day cells were fixed and permeabilized using the Image-iT® Fixation/Permeabilization Kit (R37602) according to protocol.  Cells were then