Invitrogen™

ProLong™ Diamond Antifade Mountant with SYTOX™ Deep Red

Catalog number:  P36989

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Description

ProLong Diamond Antifade Mountant is a glycerol-based liquid mountant applied directly to fluorescently labeled cells or tissue samples on microscope slides. It is formulated without DNA stain or with the blue DNA stain DAPI or with the deep red DNA stain SYTOX Deep Red. ProLong Diamond mountant is designed to provide unparalleled antifade protection across the entire visual and IR spectrums. You can use ProLong Diamond mountant with almost any fluorescent dye or fluorescent protein (e.g., GFP, RFP, mCherry) to achieve the brightest signal and lowest background, for bright, high definition fluorescent images.

Features of ProLong Diamond Antifade Mountant include:
• Protects fluorescent dyes and fluorescent protein from fading, across entire visible and IR spectrums
• Ready-to-use liquid that cures for longer-term storage
• Ideal for Alexa Fluor and traditional dyes, such as FITC and Cy3, and fluorescent proteins like GFP, RFP, and mCherry
• Mounted samples are stable for months
• Maintains fluorescence signal—little to no quenching

Learn more about ProLong Antifade mountants.

For mountants with 1.52 refractive index, optimized for oil immersion objective imaging, try curing mountant Prolong Glass Antifade Mountant or non-curing SlowFade Glass Soft-set Antifade Mountant.

ProLong Diamond Antifade Mountant comes already mixed and ready to use in a dropper bottle or 10-mL bottle with or without DNA stain. DAPI stain is excited by UV light at 360 nm when bound to DNA, with an emission maximum at 460 nm, and is detected using a DAPI traditional filter. SYTOX Deep Red stain is excited by red light at 660 nm when bound to DNA, with an emission maximum at 682 nm, and is detected using a Cy5/deep red traditional filter. Just apply a drop to the sample, add a coverslip, cure, and image. ProLong Diamond mountant is a curing mountant that forms a near-perfect optical path (1.47 RI) and enables longer-term storage of your samples, while protecting them from photobleaching. ProLong Diamond mountant does not discolor or shrink when cured and stored long term, making it possible to take high quality fluorescent images weeks or even months after mounting the slides.

Get Photobleaching Protection Across the Entire Visible and IR Spectrums
No matter which fluorescent dyes or fluorescent proteins you are using, ProLong Diamond mountant helps provide superior protection against photobleaching. It is proven to have best-in-class performance with a wide array of fluorescent dyes, including Alexa Fluor dyes, FITC, TRTC, Texas Red, Cy3, and Cy5. ProLong Diamond mountant does not quench fluorophores, and its ability to provide brighter signal, against no background, helps ensure that you get the best-quality, high resolution images for publishing.

Stable Signals During Imaging and for Longer-term Storage
ProLong Diamond mountant is the ideal option for archiving samples with fluorescent dyes or fluorescent proteins. It is also an ideal option for preserving signal strength during prolonged imaging sessions, such as repeated scanning under confocal laser scanning illumination, or with super resolution microscopy. And it's easy to use—simply remove excess water from the stained sample and mount. For immediate viewing of the sample, choose our non-curing mountant, SlowFade Diamond Antifade Mountant.

Achieve Excellent Refractive Index Matching for Crisp Images
For modern high-resolution imaging applications, refractive index (RI) matching is critical. ProLong Diamond mountant will cure to an RI of 1.47. Additionally, this curing property means you do not need to use messy acrylic resins (i.e., finger nail polish), which can damage or ruin modern microscope objectives if it comes into contact with them.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Product Line: SLOWFADE™
Product Size: 5 x 2 mL
Reagent Type: Mounting Solution
Shipping Condition: Approved for shipment at Room Temperature or on Wet Ice

Contents & storage

Contains 5 dropper bottles containing 2 mL reagent each

• Storage of product at -5 to -25°C once received is recommended.
• Protect from light.
• Stable for at least 6 months after receiving.