Electroporation Cuvettes, 0.4 cm - FAQs

Our cuvettes are known as the "Potter-style" cuvettes, and they fit most electroporators that accept a standard size cuvette.

No, one should not autoclave these cuvettes. They are gamma-irradiated to ensure sterility.

5 µg of DNA for 1 x 10e6 cells in a 0.4 cm cuvette. Electroporator settings are routinely 500 µF and 250 Volts but this may have to be optimized depending upon the make and model of the electroporator. Following electroporation, cells are plated out onto 100 mm dishes and media is changed to selective media (400 µg/ml Zeocin) after 24 hours recovery time.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

Carrier DNA is unnecessary for mammalian transfection by electroporation. Invitrogen typically uses 10 µg of target DNA WITHOUT carrier DNA.

With the exception of some fungi, 0.1 cm cuvettes are not used for electroporation of eukaryotic cells. The field strength may be set to an appropriate value but the pulse lengths will typically be too short because of the high conductance of the electroporation medium (due to the short electrode gap distance). The efficiencies of transformation are typically 0.5 - 2 logs lower. Please follow manufacturer's recommended conditions for electroporation.