The antibody I purchased from you says ''concentration not determined'' on the product label and on the product page. How do I determine the concentration?
Some antibodies are provided in non-purified formats, such as ascites fluid, tissue culture supernatant, or whole serum. These formats contain multiple proteins, which can interfere with accurate protein quantification. Traditional methods like A280 measurement or protein assays such as BCA quantify total protein but do not provide a specific concentration for the antibody in question.
Literature, such as "Antibodies" by Harlow & Lane (1988) (https://antibodiesmanual.org/index.php) (Table 8.2 - Storing and Purifying Antibodies), suggests approximate concentrations of antibodies in these solutions, as shown below. However, please note that these are rough estimates, and actual concentrations can vary based on several factors.
Serum
Total antibody concentration: 10 mg/mL
Specific antibody concentration: 0.1 to 1 mg/mL (a maximum of 10% of the antibody population is expected to be the specific antibody of interest)
Tissue Culture Supernatant (10% FBS)
Total antibody concentration: 1 mg/mL
Specific antibody concentration: 0.05 mg/mL
Tissue Culture Supernatant (serum-free)
Total antibody concentration: 0.01 to 0.05 mg/mL
Specific antibody concentration: 0.01 to 0.05 mg/mL
Ascites
Total antibody concentration: 1 to 10 mg/mL
Specific antibody concentration: 0.9 to 9 mg/mL
Find additional tips, troubleshooting help, and resources within our Antibodies and Immunoassays Support Center.
Literature, such as "Antibodies" by Harlow & Lane (1988) (https://antibodiesmanual.org/index.php) (Table 8.2 - Storing and Purifying Antibodies), suggests approximate concentrations of antibodies in these solutions, as shown below. However, please note that these are rough estimates, and actual concentrations can vary based on several factors.
Serum
Total antibody concentration: 10 mg/mL
Specific antibody concentration: 0.1 to 1 mg/mL (a maximum of 10% of the antibody population is expected to be the specific antibody of interest)
Tissue Culture Supernatant (10% FBS)
Total antibody concentration: 1 mg/mL
Specific antibody concentration: 0.05 mg/mL
Tissue Culture Supernatant (serum-free)
Total antibody concentration: 0.01 to 0.05 mg/mL
Specific antibody concentration: 0.01 to 0.05 mg/mL
Ascites
Total antibody concentration: 1 to 10 mg/mL
Specific antibody concentration: 0.9 to 9 mg/mL
Find additional tips, troubleshooting help, and resources within our Antibodies and Immunoassays Support Center.