The Invitrogen Power Blotter Select Transfer Stacks are used to transfer proteins using the Power Blotter System. They are disposable, pre-assembled stacks that come with an integrated PVDF transfer membrane for dry blotting of proteins from polyacrylamide gels. Each Power Blotter Select Transfer Stack contains filter paper, membrane, and the appropriate cathode and anode buffers incorporated into a solid gel matrix to allow fast, reliable transfer of proteins.
Features of the Power Blotter Select Transfer Stacks: • Rapid transfer—the ready-to-use transfer stack takes little setup time and protein transfers take place in as little as seven minutes • Efficient and even transfer—stacks are made with high-quality membranes that provide high binding efficiency, uniform transfer, and high detection sensitivity • Increased reproducibility—preassembled stacks provide consistent performance
PVDF membrane The PVDF membrane (0.2 µm pore size, low fluorescence) in the stack has higher binding capacity than nitrocellulose. It is pre-activated and ready for use without any pretreatment with alcohols. The membrane is compatible with commonly used detection methods such as staining, immunodetection, fluorescence, and radiolabeling. The proteins bind to the membrane through hydrophobic interactions. The protein-binding capacity is 240 µg/cm2.
Mini transfer stacks The mini-size (8 cm x 8 cm) Power Blotter Select Stacks can be used with all Invitrogen Novex, Bolt, and NuPAGE mini-size gels or equivalent.