PathoProof™ Majorû4.2 Kits for Applied Biosystems™ 7500 and 7500 Fast qPCR systems

Good-quality raw milk must meet accepted standards for chemical composition and purity as well as levels of a variety of mastitis-causing microorganisms. Identify four of the main mastitis-causing microorganisms and the Staphylococcal β-lactamase gene responsible for the penicillin resistance in Staphylococci using PathoProof Major–4.2 multiplex PCR. All targeted species are detected simultaneously, overcoming the common problem of mixed growth results in bacterial culturing.

• Ensure milk and milk products are safe and suitable for their intended uses
• Remove affected cows from the herd to ensure optimal milk quality
• Enables faster decisions on treatment
• Reduces time available for infective agents to spread to healthy herd mates
• Reduces broad spectrum antibiotic treatment
• Rapid treatment shortens inflammation time

Assays are based on real-time PCR, an accurate and sensitive technology for identification of the target DNA in the sample. Tests can be used for analyzing fresh and preserved quarter milk samples, milk recording samples, and bulk tank milk samples.

Achieve high sensitivity, even for samples that show no growth in culture. Accurate detection of viable, dead, and growth-inhibited microorganisms reduces no-growth results.

PathoProof Major-4.2 Kit identifies:

1. Mycoplasma bovis
2. Staphylococcus aureus
3. Streptococcus agalactiae
4. Streptococcus uberis

Automated sample preparation
Significantly reduce the hands-on time needed when processing larger numbers of samples with the semi-automated Thermo Scientific™ KingFisher™ Flex instrument for magnetic bead-based DNA purification and extraction, along with PathoProof Major-4.2 KingFisher Kits and qPCR reagents.

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