Long-term in vivo provision of antigen-specific T cell immunity by programming hematopoietic stem cells.
AuthorsYang L, Baltimore D,
JournalProc Natl Acad Sci U S A
PubMed ID15758071
A method to genetically program mouse hematopoietic stem cells to develop into functional CD8 or CD4 T cells of defined specificity in vivo is described. For this purpose, a bicistronic retroviral vector was engineered that efficiently delivers genes for both alpha and beta chains of T cell receptor (TCR) to ... More
Identification and characterization of undifferentiated mast cells in mouse bone marrow.
AuthorsJamur MC, Grodzki AC, Berenstein EH, Hamawy MM, Siraganian RP, Oliver C,
JournalBlood
PubMed ID15718418
Sequential immunomagnetic isolation with 2 monoclonal antibodies was used to purify and characterize an undifferentiated mast cell in adult mouse bone marrow that had not been previously recognized. This cell represents 0.02% of the cells in the bone marrow, is CD34(+), CD13(+), and c-kit(+), and does not express FcepsilonRI. However, ... More