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Prionics®-Check WESTERN Kit (12000)

Catalog number: PR12000

 Related applications: Animal Health

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The Prionics®-Check WESTERN Kit is the gold standard of rapid BSE testing and is used as a confirmatory test by most National Reference Laboratories across Europe. It owes its success to its precision and reliability. The test uses the relative protease resistance of the disease-specific prion protein PrPSc for TSE detection. Protease digestion leads to a small decrease in PrPSc protein size, whereas the normal protein PrPC is completely degraded.

The Prionics®-Check WESTERN Kit delivers a completely reliable test-result based on three independent and easily interpretable criteria: signal (PrPSc), characteristic three-band pattern BSE positive, position relative to control (PrPC).

Test procedure
1. The homogenized brain sample is incubated with an optimized reagent mixture consisting of digestion enzymes and a buffer solution. This solution degrades the normal prion protein. Only the BSE-specific prion protein remains in the test sample.
2. The proteins in the sample are then separated according to size by gel electrophoresis and transferred to a special blot membrane for detection. The BSE-specific prion protein on the membrane is detected with specific antibodies and is visualized on a digital file or on a film.
For Veterinary Use Only.


Product Line: PrioCHECK®, Prionics™
Product Size: 100 samples
Reactivity: Bovine


Manuals & protocols