Thermo Scientific™

SureTect™ Listeria monocytogenes PCR Assay

Related applications:

Industrial Microbiology

Detect Listeria monocytogenes in even the most challenging food types with the Thermo Scientific™ SureTect™ Listeria monocytogenes PCR Assay which incorporates optimized primer and probe components for highly-specific detection of L. monocytogenes. The internal amplification control confirms correct assay conditions have occurred and results can be relied on. The assay is AOAC RI and NF certified for all human foods and environmental samples for additional confidence in your results.
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The SureTect System is a unique solution combining speed and performance in an easy-to-use, cost-effective platform—giving you results you can trust.
  • Rapid actions: fast, optimized single enrichment step and automatic result interpretation
  • Simple workflow: single enrichment protocols, pre-dispensed reagents, universal PCR protocols for detection of multiple targets in the same run.
  • Intuitive, user-friendly software: reliable results, with no subjective interpretation.
  • Peace of mind: broad range of validated workflows according to AFNOR and AOAC-RI, supported by our dedicated and experienced technical teams
  • Maximum flexibility: the ability to run all assays simultaneously and the flexibility to test up to 96 samples in each run helps you operate more efficiently.
The SureTect Listeria monocytogenes PCR Assay has been validated on over 20 different matrices from broad range of food and manufacturing environments including diary, meats, seafood, vegetables and production surfaces.  All human foods and environmental materials tested during independent external validation include food types most commonly associated with contamination by Listeria monocytogenes and also those food types known to be particularly challenging to PCR detection, for example brie, smoked salmon, cantaloupe melon and salami.

  • SureTect Lysis Reagent 1 Tubes
  • SureTect Lysis Tube Caps
  • SureTect Lysis Reagent 2
  • SureTect Proteinase K
  • SureTect Listeria monocytogenes PCR Tubes
  • SureTect PCR Caps

Enriched samples are pipetted into pre-filled Lysis Tubes, along with Proteinase K and lysis reagent 2, before incubation to lyse any bacterial cells present in the sample and release their DNA into the solution. The lysate is then loaded into the SureTect Listeria monocytogenes PCR Tube to re-hydrate the preloaded PCR pellet which contains all of the necessary components and reagents for PCR, including a probe, primers and DNA template for the internal amplification control (IAC). The PCR tubes are then sealed, loaded into the Applied Biosystems™ QuantStudio™ 5 Food SafetyReal-Time PCR System with RapidFinder™ Analysis v1.0 software or the Applied Biosystems™ 7500 Fast Food Safety 96-well Real-Time PCR instrument, and the run started using the RapidFinder™ Express Software v2.0 or later. After running, interpreted results are clearly displayed and can be reported, stored, printed or downloaded as required.

As with all test methods, the source, formulation and quality of enrichment medium can influence the results. Thermo Fisher Scientific has evaluated the SureTect PCR Assays workflows with industry leading Oxoid™ and Remel™ media formulations, as well as, ISO formulations where fit for purpose.

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