Z'-LYTE® Kinase Assay Kit - Ser/Thr 1 Peptide

      Catalog number: PV3174

       Related applications: Pharma & Biopharma | Protein Assays and Analysis | Target & Lead Identification & Validation

        Contact us for support ›

      Error loading your content!

        Catalog number
      Select a plan
      Unit size
      		 Price ({{currency}}) Availability Qty
      		{{product.sku}} {{product.sku}} also known as {{product.formattedSku}} 
      		{{product.availability.message}} —
      Pro add-ons
      Customize this product ›
      Check your on-site stock ›

      Your on-site stock

      ›› {{supplyCenter.scName}}({{scProduct.stockOnHand}} In stock)
      ›› {{supplyCenter.scName}}(Out of stock)
      ›› {{supplyCenter.scName}}
      This item is not currently available on-site. Depending on your Supply Center settings you may be able to add the item to cart above else use the Order Non-Stocked Items' tab on the Supply Center home page.
      Change selected location › Order non-stocked items
      Back to top

      Description

      A coumarin and fluorescein-labeled peptide substrate which can be used for kinase screening in an antibody-free format. This peptide is based upon a porcine pyruvate liver kinase derived peptide used to study PKA substrate specificity, and containing an R-R-X-S consensus sequence, where X is any residue.
      For Research Use Only. Not for use in diagnostic procedures.

      Specifications

      Assay Type: Serine⁄Threonine Kinase Assay
      Excitation⁄Emission (nm): 405⁄465, 535
      Format: 384-well plate
      Label or Dye: Methylcoumarin
      Number of Reactions: 800 Reactions
      Product Line: Z´-LYTE®
      Product Size: 800 x 20 µL assays
      Protein or Peptide Source: porcine pyruvate liver kinase (LRRASLG)
      Readout: End Point
      References: BBRC vol. 61, pp 559-563 (1974), JBC, vol 252, p 4888 (1978)
      Substrate Properties: Peptide-Based Substrate
      Target Enzyme: Serine⁄Threonine Kinases
      Technique: FRET
      Validated Kinases: AURKA, AURKB, PRKACA, PRKG1, PRKG2, PRKX, RPS6KA4, RPS6KA5
      Shipping Condition: Dry Ice

      Contents & storage

      Z´-LYTE® Kinase Assay Kits contain all necessary reagents for performing primary or secondary screens. Most kit components should be stored at -20°C, and some kit components should be stored at -80°C. See COA for specific storage instructions.

      Documents

      Manuals & protocols