Z'-LYTE™ Kinase Assay Kit - Ser/Thr 1 Peptide

Catalog number:  PV3174

Related applications:

Protein Assays and Analysis

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Description

A coumarin and fluorescein-labeled peptide substrate which can be used for kinase screening in an antibody-free format. This peptide is based upon a porcine pyruvate liver kinase derived peptide used to study PKA substrate specificity, and containing an R-R-X-S consensus sequence, where X is any residue.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Format: 384-well plate
Assay Entry: Biochemical phospho-specific activity (no antibody)
Assay: Serine⁄Threonine Kinase Assay
Excitation/Emission: 405⁄465, 535
No. of Reactions: 800 Reactions
Source: porcine pyruvate liver kinase (LRRASLG)
References: BBRC vol. 61, pp 559-563 (1974), JBC, vol 252, p 4888 (1978)
Readout: End Point
Shipping Condition: Dry Ice
Substrate Properties: Peptide-Based Substrate
Target Entry: PRKX, PRKG2, RPS6KA5, PRKACA, PRKG1, RPS6KA4, AURKB, AURKA
Target Enzyme: Serine⁄Threonine Kinases
Technique: FRET
Validated Kinases: AURKA, AURKB, PRKACA, PRKG1, PRKG2, PRKX, RPS6KA4, RPS6KA5
Product Line: Z´-LYTE®
Label or Dye: Methylcoumarin

Contents & storage

Z´-LYTE® Kinase Assay Kits contain all necessary reagents for performing primary or secondary screens. Most kit components should be stored at -20°C, and some kit components should be stored at -80°C. See COA for specific storage instructions.