Z'-LYTE™ Kinase Assay Kit - Ser/Thr 1 Peptide
Description
A coumarin and fluorescein-labeled peptide substrate which can be used for kinase screening in an antibody-free format. This peptide is based upon a porcine pyruvate liver kinase derived peptide used to study PKA substrate specificity, and containing an R-R-X-S consensus sequence, where X is any residue.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
| Assay Type: | Serine⁄Threonine Kinase Assay |
|---|---|
| Excitation⁄Emission (nm): | 405⁄465, 535 |
| Format: | 384-well plate |
| Label or Dye: | Methylcoumarin |
| Number of Reactions: | 800 Reactions |
| Product Line: | Z´-LYTE® |
| Product Size: | 800 x 20 µL assays |
| Protein or Peptide Source: | porcine pyruvate liver kinase (LRRASLG) |
| Readout: | End Point |
| References: | BBRC vol. 61, pp 559-563 (1974), JBC, vol 252, p 4888 (1978) |
| Shipping Condition: | Dry Ice |
| Substrate Properties: | Peptide-Based Substrate |
| Target Enzyme: | Serine⁄Threonine Kinases |
| Technique: | FRET |
| Validated Kinases: | AURKA, AURKB, PRKACA, PRKG1, PRKG2, PRKX, RPS6KA4, RPS6KA5 |
Contents & storage
Z´-LYTE® Kinase Assay Kits contain all necessary reagents for performing primary or secondary screens. Most kit components should be stored at -20°C, and some kit components should be stored at -80°C. See COA for specific storage instructions.
