A coumarin and fluorescein-labeled peptide substrate which can be used for kinase screening in an antibody-free format. This peptide is based upon glycogen synthase, containing Ser-7 and a consensus seq Hyd-X-R-X-X-S⁄T-X-X, where Hyd is bulky hydrophobic, F > L > V>>A, and X is any residue.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Product Line
Z ́-LYTE™
Assay
Serine⁄Threonine Kinase Assay
Assay Entry
Biochemical phospho-specific activity (no antibody)
Excitation/Emission
405/465, 535
Format
384-well Plate
For Use With (Application)
FRET
Label or Dye
Methylcoumarin
No. of Reactions
800 Reactions
Quantity
800 x 20 μL
Readout
End Point
References
Biochem J. vol 296, pp. 843-9 (1993), J. Biol Chem, vol. 260, pp 14471-6 (1985)
Z´-LYTE™ Kinase Assay Kits contain all necessary reagents for performing primary or secondary screens. Most kit components should be stored at -20°C, and some kit components should be stored at -80°C. See COA for specific storage instructions.