Thermo Scientific™
Z'-LYTE™ Kinase Assay Kit - Ser/Thr 4 Peptide
Description
A coumarin and fluorescein-labeled peptide substrate which can be used for kinase screening in an antibody-free format. This peptide is based upon glycogen synthase, containing Ser-7 and a consensus seq Hyd-X-R-X-X-S⁄T-X-X, where Hyd is bulky hydrophobic, F > L > V>>A, and X is any residue.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
| Assay: | Serine⁄Threonine Kinase Assay |
|---|---|
| Assay Entry: | Biochemical phospho-specific activity (no antibody) |
| Excitation/Emission: | 405⁄465, 535 |
| Label or Dye: | Methylcoumarin |
| No. of Reactions: | 800 Reactions |
| Product Line: | Z´-LYTE® |
| Readout: | End Point |
| References: | Biochem J. vol 296, pp. 843-9 (1993), J. Biol Chem, vol. 260, pp 14471-6 (1985) |
| Shipping Condition: | Dry Ice |
| Substrate Properties: | Peptide-Based Substrate |
| Target Entry: | MAPK8, MAPKAPK2, MAPKAPK5, CAMK2D, MAPK9, STK22B, STK22D, CAMK2A, MAPK14, MAPK10, MAPKAPK3 |
| Target Enzyme: | Serine⁄Threonine Kinases |
| Technique: | FRET |
| Validated Kinases: | MAPKAPK2, MAPKAPK3, MAPKAPK5, STK22B, STK22D, CAMK2A, CAMK2D, MAPK8, MAPK9, MAPK10, MAPK14 |
| Source: | based on Ser 7 glycogen synthase (KKLNRTLSVA) |
| Format: | 384-well plate |
Contents & storage
Z´-LYTE® Kinase Assay Kits contain all necessary reagents for performing primary or secondary screens. Most kit components should be stored at -20°C, and some kit components should be stored at -80°C. See COA for specific storage instructions.
