Z'-LYTE® Kinase Assay Kit - Ser/Thr 4 Peptide

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Description

A coumarin and fluorescein-labeled peptide substrate which can be used for kinase screening in an antibody-free format. This peptide is based upon glycogen synthase, containing Ser-7 and a consensus seq Hyd-X-R-X-X-S⁄T-X-X, where Hyd is bulky hydrophobic, F > L > V>>A, and X is any residue.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Assay Type: Serine⁄Threonine Kinase Assay
Excitation⁄Emission (nm): 405⁄465, 535
Format: 384-well plate
Label or Dye: Methylcoumarin
Number of Reactions: 800 Reactions
Product Size: 800 x 20 µL assays
Protein or Peptide Source: based on Ser 7 glycogen synthase (KKLNRTLSVA)
Readout: End Point
References: Biochem J. vol 296, pp. 843-9 (1993), J. Biol Chem, vol. 260, pp 14471-6 (1985)
Substrate Properties: Peptide-Based Substrate
Target Enzyme: Serine⁄Threonine Kinases
Technique: FRET
Validated Kinases: CAMK2A, CAMK2D, MAPK8, MAPK9, MAPK10, MAPK14, MAPKAPK2, MAPKAPK3, MAPKAPK5, STK22B, STK22D
Shipping Condition: Dry Ice

Contents & storage

Z´-LYTE® Kinase Assay Kits contain all necessary reagents for performing primary or secondary screens. Most kit components should be stored at -20°C, and some kit components should be stored at -80°C. See COA for specific storage instructions.

Documents

Manuals & protocols