Z'-LYTE® Kinase Assay Kit - Ser/Thr 4 Peptide

      Catalog number: PV3177

       Related applications: Pharma & Biopharma | Protein Assays and Analysis | Target & Lead Identification & Validation

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      Description

      A coumarin and fluorescein-labeled peptide substrate which can be used for kinase screening in an antibody-free format. This peptide is based upon glycogen synthase, containing Ser-7 and a consensus seq Hyd-X-R-X-X-S⁄T-X-X, where Hyd is bulky hydrophobic, F > L > V>>A, and X is any residue.
      For Research Use Only. Not for use in diagnostic procedures.

      Specifications

      Assay Type: Serine⁄Threonine Kinase Assay
      Excitation⁄Emission (nm): 405⁄465, 535
      Format: 384-well plate
      Label or Dye: Methylcoumarin
      Number of Reactions: 800 Reactions
      Product Line: Z´-LYTE®
      Product Size: 800 x 20 µL assays
      Protein or Peptide Source: based on Ser 7 glycogen synthase (KKLNRTLSVA)
      Readout: End Point
      References: Biochem J. vol 296, pp. 843-9 (1993), J. Biol Chem, vol. 260, pp 14471-6 (1985)
      Substrate Properties: Peptide-Based Substrate
      Target Enzyme: Serine⁄Threonine Kinases
      Technique: FRET
      Validated Kinases: CAMK2A, CAMK2D, MAPK8, MAPK9, MAPK10, MAPK14, MAPKAPK2, MAPKAPK3, MAPKAPK5, STK22B, STK22D
      Shipping Condition: Dry Ice

      Contents & storage

      Z´-LYTE® Kinase Assay Kits contain all necessary reagents for performing primary or secondary screens. Most kit components should be stored at -20°C, and some kit components should be stored at -80°C. See COA for specific storage instructions.

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      Manuals & protocols