Z'-LYTE™ Kinase Assay Kit - Ser/Thr 6 Peptide

Back to top


A coumarin and fluorescein-labeled peptide substrate which can be used for kinase screening in an antibody-free format. This peptide contains an R-X-R-X-X-S-Hyd consensus sequence, where Hyd is a bulky hydrophobic residue, and X is any residue.
For Research Use Only. Not for use in diagnostic procedures.


Format: 384-well plate
Assay Entry: Biochemical phospho-specific activity (no antibody)
Assay: Serine⁄Threonine Kinase Assay
Excitation/Emission: 405⁄465, 535
No. of Reactions: 800 Reactions
Readout: End Point
Shipping Condition: Dry Ice
Research Category: Signal Transduction
Substrate Properties: Peptide-Based Substrate
Target Entry: SGK, SGK2, RPS6KA3, AKT2, RPS6KA1, AKT1, CLK2, PDK1, RPS6KA2, SGKL, AKT3
Target Enzyme: Serine⁄Threonine Kinases
Technique: FRET
Validated Kinases: AKT1, AKT2, AKT3, CLK2, PDK1, RPS6KA1, RPS6KA2, RPS6KA3, SGK, SGK2, SGKL
Product Line: Z´-LYTE®
Label or Dye: Methylcoumarin

Contents & storage

Z´-LYTE® Kinase Assay Kits contain all necessary reagents for performing primary or secondary screens. Most kit components should be stored at -20°C, and some kit components should be stored at -80°C. See COA for specific storage instructions.