Z'-LYTE™ Kinase Assay Kit - Ser/Thr 6 Peptide

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Description

A coumarin and fluorescein-labeled peptide substrate which can be used for kinase screening in an antibody-free format. This peptide contains an R-X-R-X-X-S-Hyd consensus sequence, where Hyd is a bulky hydrophobic residue, and X is any residue.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Assay Type: Serine⁄Threonine Kinase Assay
Excitation⁄Emission (nm): 405⁄465, 535
Format: 384-well plate
Label or Dye: Methylcoumarin
Number of Reactions: 800 Reactions
Product Line: Z´-LYTE®
Product Size: 800 x 20 µL assays
Readout: End Point
Research Category: Signal Transduction
Shipping Condition: Dry Ice
Substrate Properties: Peptide-Based Substrate
Target Enzyme: Serine⁄Threonine Kinases
Technique: FRET
Validated Kinases: AKT1, AKT2, AKT3, CLK2, PDK1, RPS6KA1, RPS6KA2, RPS6KA3, SGK, SGK2, SGKL

Contents & storage

Z´-LYTE® Kinase Assay Kits contain all necessary reagents for performing primary or secondary screens. Most kit components should be stored at -20°C, and some kit components should be stored at -80°C. See COA for specific storage instructions.