Z'-LYTE® Kinase Assay Kit - Ser/Thr 7 Peptide

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A coumarin and fluorescein-labeled peptide substrate which can be used for kinase screening in an antibody-free format. This peptide contains a PKC consensus sequence (R⁄K)1-3(X)0-2 (S⁄T) (X)0-2(R⁄K)1-3, where X is any residue.
For Research Use Only. Not for use in diagnostic procedures.


Assay Type: Serine⁄Threonine Kinase Assay
Excitation⁄Emission (nm): 405⁄465, 535
Format: 384-well plate
Label or Dye: Methylcoumarin
Number of Reactions: 800 Reactions
Product Line: Z´-LYTE®
Product Size: 800 x 20 µL assays
Protein or Peptide Source: PKC consensus sequence (R⁄K)1-3(X)0-2 S⁄T (X)0-2(R⁄K)1-3
Readout: End Point
Substrate Properties: Peptide-Based Substrate
Target Enzyme: Serine⁄Threonine Kinases
Technique: FRET
Validated Kinases: CHEK2, IRAK4, MAP3K9, MAP4K2, MAP4K4, MAP4K5, MINK1, MST4, NEK1, NEK2, NEK9, PASK, PHKG1, PHKG2, PIM1, PIM2, PKN1, PKC, ROCK1, RPS6KB1, SRPK2, STK3, STK4, STK24, STK25, TAOK2
Shipping Condition: Dry Ice

Contents & storage

Z´-LYTE® Kinase Assay Kits contain all necessary reagents for performing primary or secondary screens. Most kit components should be stored at -20°C, and some kit components should be stored at -80°C. See COA for specific storage instructions.


Manuals & protocols