Z'-LYTE® Kinase Assay Kit - Ser/Thr 7 Peptide

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Description

A coumarin and fluorescein-labeled peptide substrate which can be used for kinase screening in an antibody-free format. This peptide contains a PKC consensus sequence (R⁄K)1-3(X)0-2 (S⁄T) (X)0-2(R⁄K)1-3, where X is any residue.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Format: 384-well plate
Readout: End Point
Technique: FRET
Assay Type: Serine⁄Threonine Kinase Assay
Label or Dye: Methylcoumarin
Product Size: 800 x 20 µL assays
Target Enzyme: Serine⁄Threonine Kinases
Validated Kinases: ROCK1, RPS6KB1, SRPK2, STK3, STK4, STK24, STK25, TAOK2, NEK2, NEK9, PASK, PHKG1, PHKG2, PIM1, PIM2, PKN1, PKC, CHEK2, IRAK4, MAP3K9, MAP4K2, MAP4K4, MAP4K5, MINK1, MST4, NEK1
Number of Reactions: 800 Reactions
Substrate Properties: Peptide-Based Substrate
Protein or Peptide Source: PKC consensus sequence (R⁄K)1-3(X)0-2 S⁄T (X)0-2(R⁄K)1-3
Excitation⁄Emission (nm): 405⁄465, 535
Shipping Condition: Dry Ice

Contents & storage

Z´-LYTE® Kinase Assay Kits contain all necessary reagents for performing primary or secondary screens. Most kit components should be stored at -20°C, and some kit components should be stored at -80°C. See COA for specific storage instructions.

Documents

Manuals & protocols