A coumarin and fluorescein-labeled peptide substrate which can be used for kinase screening in an antibody-free format. This peptide contains a PKC consensus sequence (R⁄K)1-3(X)0-2 (S⁄T) (X)0-2(R⁄K)1-3, where X is any residue.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Product Line

Z´-LYTE™

Target

Serine/Threonine Kinases

For Use With (Application)

FRET

Assay

Serine/Threonine Kinase Assay

Assay Entry

Biochemical phospho-specific activity (no antibody)

Label or Dye

Methylcoumarin

Product Type

Kinase Assay Kit

Quantity

800 x 20 µL Assays

Format

384-well plate

Sample Type

PKC consensus sequence (R⁄K)1-3(X)0-2 S⁄T (X)0-2(R⁄K)1-3

Excitation/Emission

405⁄465, 535

No. of Reactions

800 Reactions

Readout

End Point

Shipping Condition

Dry Ice

Substrate

Peptide-Based Substrate

Target Entry

MAP4K5, IRAK4, PRKCQ, NEK9, PRKCD, MAP3K9, PASK, PIM2, NEK2, PRKCG, SRPK2, STK4, NEK1, MAP4K2, MINK1, CHEK2, PIM1, PKN1, PRKCA, PRKCI, TAOK2, MAP4K4, RPS6KB1, PHKG1, PRKCZ, ROCK1, PHKG2, PRKCB1, STK24, STK3, PRKCB2, STK25, PRKCE, PRKCH, MST4

Validated Kinases

ROCK1, RPS6KB1, SRPK2, STK3, STK4, STK24, STK25, TAOK2, NEK2, NEK9, PASK, PHKG1, PHKG2, PIM1, PIM2, PKN1, PKC, CHEK2, IRAK4, MAP3K9, MAP4K2, MAP4K4, MAP4K5, MINK1, MST4, NEK1

Contents & Storage

Z´-LYTE™ Kinase Assay Kits contain all necessary reagents for performing primary or secondary screens. Most kit components should be stored at -20°C, and some kit components should be stored at -80°C. See COA for specific storage instructions.

Z'-LYTE™ Kinase Assay Kit - Ser/Thr 7 Peptide

Z'-LYTE™ Kinase Assay Kit - Ser/Thr 7 Peptide

Specifications

Contents & Storage

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