Z'-LYTE™ Kinase Assay Kit - Ser/Thr 11 Peptide

Catalog number:  PV3670

Related applications:

Protein Assays and Analysis

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Description

A coumarin and fluorescein-labeled peptide substrate which can be used for kinase screening in an antibody-free format. This peptide is based upon bovine beta-casein A2, containing Ser-17.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Format: 384-well plate
Assay Entry: Biochemical phospho-specific activity (no antibody)
Assay: Serine⁄Threonine Kinase Assay
Excitation/Emission: 405⁄465, 535
No. of Reactions: 800 Reactions
Source: bovine ß-casein A2 residues 14-21 (ESLSSSEE)
References: Eur. J. Biochemistry, 186, pp.459-464 (1989)
Readout: End Point
Shipping Condition: Dry Ice
Substrate Properties: Peptide-Based Substrate
Target Entry: IKBKE, CSNK1D, CSNK2A1, FRAP1 (mTOR), CSNK1E, CSNK2A2, CSNK1A1
Target Enzyme: Serine⁄Threonine Kinases
Technique: FRET
Validated Kinases: CSNK1A1, CSNK1D, CSNK1E, CSNK2A1 , CSNK2A2, IKBKE, FRAP1 (mTOR)
Product Line: Z´-LYTE®
Label or Dye: Methylcoumarin

Contents & storage

Z´-LYTE® Kinase Assay Kits contain all necessary reagents for performing primary or secondary screens. Most kit components should be stored at -20°C, and some kit components should be stored at -80°C. See COA for specific storage instructions.