Z'-LYTE™ Kinase Assay Kit - Ser/Thr 11 Peptide

      Catalog number:  PV3670

       Related applications: Pharma & Biopharma | Protein Assays and Analysis | Target & Lead Identification & Validation

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      Description

      A coumarin and fluorescein-labeled peptide substrate which can be used for kinase screening in an antibody-free format. This peptide is based upon bovine beta-casein A2, containing Ser-17.
      For Research Use Only. Not for use in diagnostic procedures.

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      Specifications

      Assay Type: Serine⁄Threonine Kinase Assay
      Excitation⁄Emission (nm): 405⁄465, 535
      Format: 384-well plate
      Label or Dye: Methylcoumarin
      Number of Reactions: 800 Reactions
      Product Line: Z´-LYTE®
      Product Size: 800 x 20 µL assays
      Protein or Peptide Source: bovine ß-casein A2 residues 14-21 (ESLSSSEE)
      Readout: End Point
      References: Eur. J. Biochemistry, 186, pp.459-464 (1989)
      Substrate Properties: Peptide-Based Substrate
      Target Enzyme: Serine⁄Threonine Kinases
      Technique: FRET
      Validated Kinases: CSNK1A1, CSNK1D, CSNK1E, CSNK2A1 , CSNK2A2, IKBKE, FRAP1 (mTOR)
      Shipping Condition: Dry Ice

      Contents & storage

      Z´-LYTE® Kinase Assay Kits contain all necessary reagents for performing primary or secondary screens. Most kit components should be stored at -20°C, and some kit components should be stored at -80°C. See COA for specific storage instructions.

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