Thermo Scientific™

Z'-LYTE™ Kinase Assay Kit - Ser/Thr 14 Peptide

Catalog number:  PV3796

Related applications:

Protein Assays and Analysis

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Description

A coumarin and fluorescein-labeled serine/threonine peptide substrate which can be used for kinase screening in an antibody-free format. This peptide is derived from a peptide substrate for PAK.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Assay: Serine/Threonine Kinase Assay
Assay Entry: Biochemical phospho-specific activity (no antibody)
Excitation/Emission: 405/465, 535
Label or Dye: Methylcoumarin
No. of Reactions: 800 Reactions
Product Line: Z ́-LYTE™
Readout: End Point
References: Biochem. J. vol 366, pp. 977-81 (2002)
Shipping Condition: Dry Ice
Substrate Properties: Peptide-Based Substrate
Target Entry: PAK1
Target Enzyme: Serine/Threonine Kinases
Technique: FRET
Validated Kinases: PAK1
Source: generic peptide for S/T kinases, PAK included (RRRLSFAEPG)
Format: 384-well plate

Contents & storage

Z ́-LYTE™ Kinase Assay Kits contain all necessary reagents for performing primary or secondary screens. Most kit components should be stored at -20°C, and some kit components should be stored at -80°C. See COA for specific storage instructions.