Z'-LYTE® Kinase Assay Kit - Tyrosine 7 Peptide

Catalog number: PV4305

 Related applications: Protein Assays and Analysis | Target & Lead Identification & Validation

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Description

A coumarin and fluorescein-labeled peptide substrate which can be used for kinase screening in an antibody-free format. This peptide is based upon LAT, containing Tyr-200.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Format: 384-well plate
Readout: End Point
Technique: FRET
Assay Type: Tyrosine Kinase Assay
References: Biochem. J. 356 p 461-71 (2001)
Label or Dye: Methylcoumarin
Product Size: 800 x 20 µL assays
Target Enzyme: Tyrosine Kinases
Validated Kinases: ZAP70
Number of Reactions: 800 Reactions
Substrate Properties: Peptide-Based Substrate
Protein or Peptide Source: LAT, residues 195 to 208, centering on Y-200 (ESIDDYVNVPESGES)
Excitation⁄Emission (nm): 405⁄465, 535
Shipping Condition: Dry Ice

Contents & storage

Z´-LYTE® Kinase Assay Kits contain all necessary reagents for performing primary or secondary screens. See COA for specific storage instructions Most kit components should be stored at -20°C, and some kit components should be stored at -80°C. See COA for specific storage instructions.

Documents

Manuals & protocols