Thermo Scientific™

Z'-LYTE™ Kinase Assay Kit - Tyrosine 7 Peptide

Catalog number:  PV4305

Related applications:

Protein Assays and Analysis

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Description

A coumarin and fluorescein-labeled peptide substrate which can be used for kinase screening in an antibody-free format. This peptide is based upon LAT, containing Tyr-200.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Assay: Tyrosine Kinase Assay
Assay Entry: Biochemical phospho-specific activity (no antibody)
Excitation/Emission: 405⁄465, 535
Label or Dye: Methylcoumarin
No. of Reactions: 800 Reactions
Product Line: Z´-LYTE®
Readout: End Point
References: Biochem. J. 356 p 461-71 (2001)
Shipping Condition: Dry Ice
Substrate Properties: Peptide-Based Substrate
Target Entry: ZAP70
Target Enzyme: Tyrosine Kinases
Technique: FRET
Validated Kinases: ZAP70
Source: LAT, residues 195 to 208, centering on Y-200 (ESIDDYVNVPESGES)
Format: 384-well plate

Contents & storage

Z´-LYTE® Kinase Assay Kits contain all necessary reagents for performing primary or secondary screens. See COA for specific storage instructions Most kit components should be stored at -20°C, and some kit components should be stored at -80°C. See COA for specific storage instructions.