A coumarin and fluorescein-labeled peptide substrate which can be used for kinase screening in an antibody-free format. This peptide is based upon LAT, containing Tyr-200.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Product Line
Z ́-LYTE™
Assay
Tyrosine Kinase Assay
Assay Entry
Biochemical phospho-specific activity (no antibody)
Excitation/Emission
405/465, 535
Format
384-well Plate
For Use With (Application)
FRET
Label or Dye
Methylcoumarin
No. of Reactions
800 Reactions
Quantity
800 x 20 μL
Readout
End Point
References
Biochem. J. 356 p 461-71 (2001)
Shipping Condition
Dry Ice
Source
Based on LAT, Residues 195 to 208, Centering Y-200 (ESIDDYVNVPESGES)
Substrate Properties
Peptide-Based Substrate
Target Entry
ZAP70
Target Enzyme
Tyrosine Kinases
Validated Kinases
ZAP70
Contents & Storage
Z´-LYTE™ Kinase Assay Kits contain all necessary reagents for performing primary or secondary screens. See COA for specific storage instructions Most kit components should be stored at -20°C, and some kit components should be stored at -80°C. See COA for specific storage instructions.