Z'-LYTE® Kinase Assay Kit - Ser/Thr 17 Peptide

Catalog number: PV4316

 Related applications: Protein Assays and Analysis | Target & Lead Identification & Validation

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Description

A coumarin and fluorescein-labeled peptide substrate which can be used for kinase screening in an antibody-free format. This peptide is based upon glycogen synthase, containing Ser-7.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Format: 384-well plate
Readout: End Point
Technique: FRET
Assay Type: Serine⁄Threonine Kinase Assay
References: Biochem J. vol 296, pp. 843-9 (1993)
Label or Dye: Methylcoumarin
Product Size: 800 x 20 µL assays
Target Enzyme: Serine⁄Threonine Kinases
Validated Kinases: CAMK2B, DCAMKL2 , MELK, NEK4, PRKCN , PRKD1, PRKD2
Number of Reactions: 800 Reactions
Substrate Properties: Peptide-Based Substrate
Protein or Peptide Source: similar to Ser-7 of glycogen synthase KKLNRTLSVA (KKLNRTLSFAEPG)
Excitation⁄Emission (nm): 405⁄465, 535
Shipping Condition: Dry Ice

Contents & storage

Z´-LYTE® Kinase Assay Kits contain all necessary reagents for performing primary or secondary screens. Most kit components should be stored at -20°C, and some kit components should be stored at -80°C. See COA for specific storage instructions.

Documents

Manuals & protocols