Assay: | Serine⁄Threonine Kinase Assay |
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Assay Entry: | Biochemical phospho-specific activity (no antibody) |
Excitation/Emission: | 405⁄465, 535 |
Label or Dye: | Methylcoumarin |
No. of Reactions: | 800 Reactions |
Product Line: | Z´-LYTE® |
Readout: | End Point |
References: | Biochem J. vol 296, pp. 843-9 (1993) |
Shipping Condition: | Dry Ice |
Substrate Properties: | Peptide-Based Substrate |
Target Entry: | CAMK2B, PRKCN, PRKD2, DCAMKL2, PRKD1, NEK4, MELK |
Target Enzyme: | Serine⁄Threonine Kinases |
Technique: | FRET |
Validated Kinases: | CAMK2B, DCAMKL2 , MELK, NEK4, PRKCN , PRKD1, PRKD2 |
Source: | similar to Ser-7 of glycogen synthase KKLNRTLSVA (KKLNRTLSFAEPG) |
Format: | 384-well plate |