A coumarin and fluorescein-labeled peptide substrate which can be used for kinase screening in an antibody-free format. This peptide is based upon an optimized synthetic peptide substrate for DYRK1A, and containing an R-P-X-(S⁄T)-P consensus sequence, where X is any residue.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Product Line
Z´-LYTE™
Target
Serine/Threonine Kinases
For Use With (Application)
FRET
Assay
Serine/Threonine Kinase Assay
Assay Entry
Biochemical phospho-specific activity (no antibody)
Label or Dye
Methylcoumarin
Product Type
Kinase Assay Kit
Quantity
800 x 20 µL Assays
Format
384-well plate
Sample Type
RRFRPASPLRGPP
Excitation/Emission
405⁄465, 535
No. of Reactions
800 Reactions
References
JBC 275, pg 2431 (2000)
Readout
End Point
Shipping Condition
Dry Ice
Substrate
Peptide-Based Substrate
Target Entry
DYRK1A, HIPK4, SRPK1, DYRK1B, CLK3, STK23
Validated Kinases
CLK3, DYRK1A, DYRK1B, HIPK4, SRPK1, STK23
Contents & Storage
Z´-LYTE™ Kinase Assay Kits contain all necessary reagents for performing primary or secondary screens. Most kit components should be stored at -20°C, and some kit components should be stored at -80°C. See COA for specific storage instructions.