This kit contains a goat Tb-anti-GST antibody—an equivalent kit containing a rabbit-sourced antibody is also available.

LanthaScreen® TR-FRET ERR gamma Coactivator Assay Kit, goat

Catalog number: PV4408

 Related applications: Pharma & Biopharma | Target & Lead Identification & Validation

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Description

This kit contains Goat Tb-Anti-GST antibody; other kit components are the same as kit A15121:

The LanthaScreen® TR-FRET Estrogen Related Receptor (ERR) gamma Coactivator Assay Kit provides a sensitive and robust method for high-throughput screening of potential ERR gamma ligands as antagonists/inverse agonists of coactivator displacement. The homogeneous mix-and-read assay format results in a ligand EC50 composite value representing the amount of ligand required to bind to receptor, effect a conformational change, and displace coactivator peptide (Figure 1).

How it works

The LanthaScreen® TR-FRET ERR gamma Coactivator Assay Kit includes a terbium (Tb)-labeled anti-GST antibody, a fluorescein-labeled coactivator peptide, and a human ERR gamma ligand-binding domain (ERR gamma-LBD) that is tagged with glutathione-S-transferase (GST). To assay, ERR gamma-LBD is added to test compounds, followed by addition of a mixture of the fluorescein-coactivator peptide and Tb-anti- GST antibody. After room temperature incubation, the TR-FRET 520:495 emission ratio is calculated and used to determine the EC50 from a dose response curve of the compound.

Contents and Storage:

The LanthaScreen® TR-FRET ERR gamma Coactivator Assay Kit contains ERR gamma-LBD (GST) protein, fluorescently labeled PGC1a coactivator peptide, Tb-anti-GST antibody, and buffers. Store components as indicated in the assay protocol (-80°C, -20°C, or +4°C).
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Detection Method: Fluorescent
For Use With (Equipment): Microplate Reader
Format: 384-well plate
Gene ID: 2104
Label or Dye: Tb (Terbium)
Product Size: 800 x 20 µL assays
Readout: End Point
Technique: TR-FRET
Validated Application: Co-Factor Interaction Assay
Shipping Condition: Dry Ice

Contents & storage

Store in ultra-cold freezer (-68 to -85°C).

Documents

Manuals & protocols