Z'-LYTE™ Kinase Assay Kit - Ser/Thr 22 Peptide

Catalog number:  PV4572

Related applications:

Pharma & Biopharma

| Protein Assays and Analysis

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Description

A coumarin and fluorescein-labeled peptide substrate which can be used for kinase screening in an antibody-free format. This peptide is based upon CDK7, containing Ser-161.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Assay Type: Serine⁄Threonine Kinase Assay
Excitation⁄Emission (nm): 405⁄465, 535
Format: 384-well plate
Label or Dye: Methylcoumarin
Number of Reactions: 800 Reactions
Product Line: Z´-LYTE®
Product Size: 800 x 20 µL assays
Protein or Peptide Source: CDK7 (GLAKSFGSPNRA)
Readout: End Point
References: JBC v 277:27839-27849 (2002)
Shipping Condition: Dry Ice
Substrate Properties: Peptide-Based Substrate
Target Enzyme: Serine⁄Threonine Kinases
Technique: FRET
Validated Kinases: NEK6, NEK7

Contents & storage

Z´-LYTE® Kinase Assay Kits contain all necessary reagents for performing primary or secondary screens. Most kit components should be stored at -20°C, and some kit components should be stored at -80°C. See COA for specific storage instructions.