A coumarin and fluorescein-labeled peptide substrate which can be used for kinase screening in an antibody-free format. This peptide is based upon CDK7, containing Ser-161.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Product Line
Z ́-LYTE™
Assay
Serine⁄Threonine Kinase Assay
Assay Entry
Biochemical phospho-specific activity (no antibody)
Excitation/Emission
405/465, 535
Format
384-well Plate
For Use With (Application)
FRET
Label or Dye
Methylcoumarin
No. of Reactions
800 Reactions
Quantity
800 x 20 μL
Readout
End Point
References
JBC v 277:27839-27849 (2002)
Shipping Condition
Dry Ice
Source
Based on CDK7 (GLAKSFGSPNRA)
Substrate Properties
Peptide-Based Substrate
Target Entry
NEK7, NEK6
Target Enzyme
Serine⁄Threonine Kinases
Validated Kinases
NEK6, NEK7
Contents & Storage
Z´-LYTE™ Kinase Assay Kits contain all necessary reagents for performing primary or secondary screens. Most kit components should be stored at -20°C, and some kit components should be stored at -80°C. See COA for specific storage instructions.
