This kit contains a goat Tb-anti-GST antibody—an equivalent kit containing a rabbit-sourced antibody is also available.

LanthaScreen® TR-FRET FXR Coactivator Assay Kit, goat

Catalog number: PV4833

 Related applications: Target & Lead Identification & Validation

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Description

This kit contains Goat Tb-Anti-GST antibody; other kit components are the same as kit A15140:

The LanthaScreen® TR-FRET FXR (farnesoid X receptor) Coactivator Assay Kit provides a sensitive and robust method for high-throughput screening of potential FXR ligands as agonists of coactivator recruitment. The kit uses a terbium (Tb)-labeled anti-GST antibody, a fluorescein- labeled coactivator peptide, and an FXR ligand-binding domain (FXR-LBD) that is tagged with glutathione-S-transferase (GST), in a homogeneous, mix-and-read assay format.

To assay:
When running the LanthaScreen® TR-FRET FXR coactivator assay in agonist mode, FXR-LBD is added to ligand test compounds followed by addition of a mixture of the fluorescein-coactivator peptide and Tb-labeled anti-GST antibody. After an incubation period at room temperature, the 520 nm/495 nm TR-FRET ratio is calculated and used to determine the EC50 from a dose response curve of the compound. Based on the biology of the FXR-coactivator peptide interaction, the ligand EC50 is a composite value representing the amount of ligand required to bind to receptor, effect a conformational change, and recruit coactivator peptide (Figure 1).
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Format: 384-well plate
Gene ID: 9971
Readout: End Point
Technique: TR-FRET
Label or Dye: Tb (Terbium)
Product Size: 800 x 20 µL assays
Detection Method: Fluorescent
Validated Application: Co-Factor Interaction Assay
For Use With (Equipment): Microplate Reader
Shipping Condition: Dry Ice

Contents & storage

The LanthaScreen® TR-FRET FXR Coactivator Assay Kit contains FXR-LBD (GST) protein, fluorescently labeled SRC2-2 coactivator peptide, Tb-labeled anti-GST antibody, and buffers.

Store components as indicated in the assay protocol (-80°C, -20°C, or +4°C).

Documents

Manuals & protocols