This kit contains a goat Tb-anti-GST antibody—an equivalent kit containing a rabbit-sourced antibody is also available.

LanthaScreen® TR-FRET PXR (SXR) Competitive Binding Assay Kit, goat

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Description

This kit contains Goat Tb-Anti-GST antibody; other kit components are the same as kit A15142:

The LanthaScreen® TR-FRET PXR (SXR) Competitive Binding Assay Kit provides a sensitive and robust method for high-throughput screening (HTS) of ligands for the pregnane X receptor (PXR), also known as the steroid and xenobiotic receptor (SXR). The kit uses a terbium (Tb)-labeled anti-GST antibody, a fluorescent small-molecule PXR ligand (Fluormone™ PXR (SXR) Green), and a human PXR (SXR) ligand-binding domain that is tagged with glutathione S-transferase (GST) in a homogeneous mix-and-read assay format.

To assay:
When running the LanthaScreen® TR-FRET PXR (SXR) competitive binding assay, a Tb-labeled anti-GST antibody is used to indirectly label the receptor by binding to its GST tag. Competitive ligand binding is detected by a test compound’s ability to displace a fluorescent ligand (tracer) from the receptor, which results in a loss of FRET signal between the Tb-anti-GST antibody and the tracer (Figure 1). This type of binding assay is analogous to radioligand-based assays, except that it eliminates handling of radioactivity and enables a homogeneous, “addition-only " format.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Detection Method: Fluorescent
For Use With (Equipment): Microplate Reader
Format: 384-well plate
Gene ID: 8856
Label or Dye: Tb (Terbium)
Product Size: 800 x 20 µL assays
Readout: End Point
Technique: TR-FRET
Validated Application: Competitive Binding Assay
Shipping Condition: Dry Ice

Contents & storage

Store in ultra-cold freezer (-68 to -85°C).

Documents

Manuals & protocols