LanthaScreen™ TR-FRET PXR (SXR) Competitive Binding Assay Kit, goat

Catalog number: PV4839

LanthaScreen™ TR-FRET PXR (SXR) Competitive Binding Assay Kit, goat

Catalog number: PV4839
Catalog Number
PV4839
Unit Size
800 x 20 µL assays
Price (EUR)
Price: 1.868,00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (EUR)AvailabilityQuantity
PV4839800 x 20 µL assays
Price: 1.868,00
Your Price:
***
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This kit contains Goat Tb-Anti-GST antibody; other kit components are the same as kit A15142:

The LanthaScreen™ TR-FRET PXR (SXR) Competitive Binding Assay Kit provides a sensitive and robust method for high-throughput screening (HTS) of ligands for the pregnane X receptor (PXR), also known as the steroid and xenobiotic receptor (SXR). The kit uses a terbium (Tb)-labeled anti-GST antibody, a fluorescent small-molecule PXR ligand (Fluormone™ PXR (SXR) Green), and a human PXR (SXR) ligand-binding domain that is tagged with glutathione S-transferase (GST) in a homogeneous mix-and-read assay format.

To assay:
When running the LanthaScreen™ TR-FRET PXR (SXR) competitive binding assay, a Tb-labeled anti-GST antibody is used to indirectly label the receptor by binding to its GST tag. Competitive ligand binding is detected by a test compound’s ability to displace a fluorescent ligand (tracer) from the receptor, which results in a loss of FRET signal between the Tb-anti-GST antibody and the tracer (Figure 1). This type of binding assay is analogous to radioligand-based assays, except that it eliminates handling of radioactivity and enables a homogeneous, “addition-only ” format.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Conjugate
Tb (Terbium)
Detection Method
Fluorescent
For Use With (Equipment)
Microplate Reader
Assay Entry
Biochemical competitive binding
Gene ID (Entrez)
8856
Readout
End Point
Shipping Condition
Dry Ice
Target Entry
NR1I2, PXR(SXR)
Form
384-well Plate
Product Line
LanthaScreen™
For Use With (Application)
Competitive Binding Assay, TR-FRET
Ligand
PXR
Product Type
TR-FRET PXR (SXR) Competitive Binding Assay Kit

Contents & Storage

Store in ultra-cold freezer (-68 to -85°C).