Thermo Scientific™

Kinase Tracer 178, 25 µM

Catalog number:  PV5593

Related applications:

Pharma & Biopharma

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Description

In the LanthaScreen™ Eu Kinase Binding Assay, binding of an Alexa Fluor™ conjugate or “tracer" such as Kinase Tracer 178 to a kinase is detected by addition of a Eu-labeled anti-tag antibody. Binding of the tracer & antibody to a kinase results in a high degree of FRET, whereas displacement of the tracer with a kinase inhibitor results in a loss of FRET. Unlike many kinase activity assays, this assay is a simple mix and read assay, with no development steps.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Format: 384-well plate
Assay Entry: Biochemical binding/kinetic
Assay: Kinase Binding Assay
Readout: Kinetic
Shipping Condition: Dry Ice
Target Entry: PTK6, DDR2, BMPR1A, RAF1 (cRAF) Y340D Y341D, ACVR1B, WNK2, TEC, DDR1, EPHB4, ERBB4, KIT V654A, TGFBR1, NLK, TGFBR2, EPHB3, BRAF V599E, BRAF, ACVR2B, ACVR2A
Target Enzyme: Kinases (Multiple)
Technique: TR-FRET
For Use With (Equipment): Microplate Reader
Label or Dye: Alexa Fluor™ 647

Contents & storage

Store at -20°C.