This PolarScreen™ PPARγ-Competitor Assay Kit, Green replaces a previous version of the kit (catalog number PV3355). Both versions perform similarly. The new kit contains a GST-tagged PPARγ-LBD while the old kit was His-tagged.

PolarScreen™ PPARγ-Competitor Assay Kit, Green

Catalog number:  PV6136

 Related applications:

Pharma & Biopharma

| Target & Lead Identification & Validation

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The PPARγ (Peroxisome Proliferator-Activated Receptor-gamma) Competitor Assay, Green uses the human-derived recombinant PPARγ ligand-binding domain (PPARγ-LBD) tagged with an N-terminal GST-tag and a novel, tight-binding, selective fluorescent PPARγ ligand (Fluormone™ PPARγ Green) in a homogenous, mix-and-read assay format. PPARγ-LBD/Fluormone™ PPARγ Green complex produces high polarization. This complex is then added to individual test compounds in microwell plates. Competitors displace the fluorescent Fluormone™ PPARγ Green Ligand from the PPARγ-LBD/Fluormone™ PPARγ Green complex, causing the fluorescent ligand to tumble rapidly during its fluorescence lifetime, resulting in a low polarization value. Noncompetitors will not displace the fluorescent ligand from the complex, so the polarization value remains high. The shift in polarization value in the presence of the test compounds is used to determine relative affinity of test compounds for the PPARγ-LBD.
WARNING: Reproductive Harm -
For Research Use Only. Not for use in diagnostic procedures.


Color: Green
Detection Method: Fluorescent
Excitation⁄Emission (nm): 485⁄535
For Use With (Equipment): Microplate Reader
Format: 384-well plate
Gene ID: 5468
Product Line: PolarScreen™
Product Size: 800 x 20 µL
Readout: End Point
Technique: Fluorescence Polarization
Validated Application: Competitive Binding Assay
Shipping Condition: Dry Ice

Contents & storage

PV3356 — Fluormone™ PPARγ Green (-20°C)
PV4546 — PPARγ-LBD (-80°C)
PV3358 — PPARγ Green Screening Buffer (room temperature)
P2325 — 1M DTT (-80°C or -20°C)


Manuals & protocols