This PolarScreen™ PPARγ-Competitor Assay Kit, Green replaces a previous version of the kit (catalog number PV3355). Both versions perform similarly. The new kit contains a GST-tagged PPARγ-LBD while the old kit was His-tagged.

PolarScreen™ PPARγ-Competitor Assay Kit, Green

Catalog number: PV6136

 Related applications: Target & Lead Identification & Validation

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Description

The PPARγ (Peroxisome Proliferator-Activated Receptor-gamma) Competitor Assay, Green uses the human-derived recombinant PPARγ ligand-binding domain (PPARγ-LBD) tagged with an N-terminal GST-tag and a novel, tight-binding, selective fluorescent PPARγ ligand (Fluormone™ PPARγ Green) in a homogenous, mix-and-read assay format. PPARγ-LBD/Fluormone™ PPARγ Green complex produces high polarization. This complex is then added to individual test compounds in microwell plates. Competitors displace the fluorescent Fluormone™ PPARγ Green Ligand from the PPARγ-LBD/Fluormone™ PPARγ Green complex, causing the fluorescent ligand to tumble rapidly during its fluorescence lifetime, resulting in a low polarization value. Noncompetitors will not displace the fluorescent ligand from the complex, so the polarization value remains high. The shift in polarization value in the presence of the test compounds is used to determine relative affinity of test compounds for the PPARγ-LBD.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Color: Green
Format: 384-well plate
Gene ID: 5468
Readout: End Point
Technique: Fluorescence Polarization
Product Size: 800 x 20 µL
Detection Method: Fluorescent
Validated Application: Competitive Binding Assay
For Use With (Equipment): Microplate Reader
Excitation⁄Emission (nm): 485⁄535
Shipping Condition: Dry Ice

Contents & storage

PV3356 — Fluormone™ PPARγ Green (-20°C)
PV4546 — PPARγ-LBD (-80°C)
PV3358 — PPARγ Green Screening Buffer (room temperature)
P2325 — 1M DTT (-80°C or -20°C)

Documents

Manuals & protocols