Quantum dot targeting with lipoic acid ligase and HaloTag for single-molecule imaging on living cells.
AuthorsLiu DS, Phipps WS, Loh KH, Howarth M, Ting AY,
JournalACS Nano
PubMed ID23181687
'We present a methodology for targeting quantum dots to specific proteins on living cells in two steps. In the first step, Escherichia coli lipoic acid ligase (LplA) site-specifically attaches 10-bromodecanoic acid onto a 13 amino acid recognition sequence that is genetically fused to a protein of interest. In the second ... More
Optical coding of mammalian cells using semiconductor quantum dots.
AuthorsMattheakis LC, Dias JM, Choi YJ, Gong J, Bruchez MP, Liu J, Wang E
JournalAnal Biochem
PubMed ID15051536
Cell-based assays are widely used to screen compounds and study complex phenotypes. Few methods exist, however, for multiplexing cellular assays or labeling individual cells in a mixed cell population. We developed a generic encoding method for cells that is based on peptide-mediated delivery of quantum dots (QDs) into live cells. ... More
Qdot nanobarcodes for multiplexed gene expression analysis.
AuthorsEastman PS, Ruan W, Doctolero M, Nuttall R, de Feo G, Park JS, Chu JS, Cooke P, Gray JW, Li S, Chen FF
JournalNano Lett
PubMed ID16683851
We report a quantum dot (Qdot) nanobarcode-based microbead random array platform for accurate and reproducible gene expression profiling in a high-throughput and multiplexed format. Four different sizes of Qdots, with emissions at 525, 545, 565, and 585 nm are mixed with a polymer and coated onto the 8-mum-diameter magnetic microbeads ... More