Yeast Nitrogen Base

Yeast Nitrogen Base - FAQs

View additional product information for Yeast Nitrogen Base - FAQs (Q30007)

2 product FAQs found

What are the components and concentrations for 1X YNB (yeast nitrogen base)?

For the pouches sold as Cat. No. Q30007, each pouch contains reagents to prepare 500 mL of 10X YNB for Pichia media or 1,000 mL of 10X YNB for S. cerevisiae media (since Pichia media is 2X what is used for S. cerevisiae). Below is the formulation for 1X YNB:

Ammonium sulfate, 5 g
Biotin, 0.002 mg
Calcium pantothenate, 0.4 mg
Folic acid, 0.002 mg
Inositol, 2 mg
Niacin, 0.4 mg
Para-aminobenzoic acid, 0.2 mg
Pyridoxine HCl, 0.4 mg
Riboflavin, 0.2 mg
Thiamine HCl, 0.4 mg
Boric acid, 0.5 mg
Copper sulfate, 0.4 mg
Potassium iodide, 0.1 mg
Ferric chloride.6H2O, 0.2 mg
Manganese sulfate.H2O, 0.4 mg
Sodium molybdate.2H2O, 0.2 mg
Zinc sulfate.H2O, 0.4 mg
Potassium phosphate monobasic, 1 g
Magnesium sulfate, 0.5 g
Sodium chloride, 0.1 g
Calcium chloride, 0.1 mg

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.

Can YNB medium (with ammonium sulfate, without amino acids) be autoclaved instead of filter-sterilized?

Yes, YNB medium with ammonium sulfate and without amino acids can be autoclaved for sterilization.

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.