This QuantiGene Sample Processing Kit for cultured cells contains reagents and instructions for the preparation of cell lysates for use in QuantiGene Singleplex and QuantiGene Plex assays for RNA targets and QuantiGene Plex DNA assays for DNA targets.
• Easy to follow instructions • Preserves the integrity of the RNA/DNA without degradation • Choose the method that matches your cultured cell sample
What you get The QuantiGene Sample Processing Kit for cultured cells contains our proprietary lysis mixture, proteinase K, and detailed instructions for preparing cell lysates from cultured cells.
Success in cell lysis Key factors that influence the complete lysis of the cells include: • The lysis mixture • Proteinase K • Vortexing or pipetting of the sample • Storage of the lysates at -80°C • Incubation at 50°C
The protocols provided with the QuantiGene Sample Processing Kit for cultured cells use a combination of these factors to ensure complete lysis of the samples. In addition, the lysis mixture provides the proper ionic conditions for the overnight hybridization in the QuantiGene assays.
For Research Use Only. Not for use in diagnostic procedures.
Contents & Storage
• 75 mL Lysis Mixture, store at 15–30°C • 750 µL Proteinase K (50 µg/µL), store at –20°C