Chocolate Agar w/Bacitracin

Isolate Haemophilus species from respiratory specimens containing mixed flora with Thermo Scientific™ Remel™ Chocolate Agar w/Bacitracin. Isolation and identification of Haemophilus spp is often hindered by overgrowth with commensal microbial flora. Hoving and Aandahl reported improved recovery of Haemophilus on Chocolate Agar with Bacitracin as compared to isolation on Sheep Blood Agar with a streak of Staphylococcus aureus.

Hemoglobin in the Chocolate Agar formulation provides the X factor, and GCHI Enrichment provides the V factor required for growth of fastidious organisms. The presence of corn-starch ensures that toxic metabolites produced Haemophilus by are absorbed. Bacitracin is a selective agent which is inhibitory to most strains of streptococci, staphylococci, Neiseeria spp., and Micococcus spp.

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Remel™ and Oxoid™ products are now part of the Thermo Scientific brand.