Haemophilus Isolation Agar w/bacitracin and horse blood

Selectively isolate Haemophilus species from respiratory specimens with Thermo Scientific™ Remel™ Haemophilus Isolation Agar w/bacitracin and horse blood. Haemophilus influenzae, a fastidious gram-negative bacillus, is a common cause of serious diseases of the upper and lower respiratory tract¹.

Haemophilus Isolation Agar w/bacitracin and horse blood is a solid media recommended for use in qualitative procedures for selective isolation of Haemophilus spp. from respiratory specimens.

• Selective: Kanamycin and vancomycin antibiotics inhibit gram-positive organisms and facultative anaerobic bacteria. Neomycin stimulates the growth of gram-negative anaerobes, especially Bacteriodes spp. Colistin and nalidixic inhibit the growth of most gram-negative bacilli.
• Enriched medium: The presence of intact erythrocytes in horse blood enables differentiation of hemolytic and nonhemolytic Haemophilus spp. Horse blood, unlike sheep blood, does not contain V factor-inactivating enzymes.
• Ready-to-use: Convenience of prepared media.

Beef heart Infusion and casein peptone supply the nutritional requirements for all but the fastidious bacteria. Horse sheep supplies both the X and V factors, which are growth requirements for certain organisms, such as Haemophilus influenzae. Horse blood also serves to distinguish the hemolytic species of Haemophilus from non-hemolytic Haemophilus species. Bacitracin inhibits normal flora found in respiratory cultures, particularly Neisseria spp.⁴.

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