Rapidly isolate and presumptively identify Extended Spectrum Beta-Lactamase (ESBL)-producing Enterobacteriaceae whilst selectively inhibiting non-ESBL Enterobacteriaceae, and some AmpC organisms and other non-ESBL flora, with Thermo ScientificTM SpectraTM ESBL Medium. The FDA-cleared chromogenic medium facilitates earlier intervention through enabling positive screen results in 18 to 24 hours directly from perirectal swabs or fresh stool specimens. The medium reduces the amount of confirmatory work-up and the costs incurred due to unnecessary isolation and additional testing.
Extended Spectrum β Lactamases are enzymes capable of conferring bacterial resistance to penicillins, cephalosporins, and aztreonam by hydrolysis of these antibiotics 1,2. Production of ESBLs is a significant resistance-mechanism that impedes the antimicrobial treatment of infections caused by Enterobacteriaceae and is a serious threat to the currently available antibiotic armory 3.
Spectra ESBL Medium supports HAI surveillance programs through fast isolation and presumptive identification of ESBL-producing bacteria in clinical samples.
Easy-to-read: clearly differentiates ESBL-producing organisms by their blue/turquoise-green or pink colonies.
Facilitates early intervention: fast, accurate results support infection prevention and control.
High specificity: reduces costs associated with unnecessary isolation and additional testing.
Streamlined workflow: screen positive within 18 to 24 hours directly from perirectal swabs or fresh stool specimens.
Ready-to-use: convenience of prepared media.
First FDA-cleared chromogenic media for the presumptive identification of Proteus.
A mixture of chromogens forms a substrate for two enzymes: β-galactosidase and glucuronidase that are differentially expressed in different species of bacteria resulting in blue/turquoise-green or pink colonies. Other ESBL-producing organisms that do not utilize the chromogenic substrates may produce tan colonies through deamination of tryptophan. Non-target organisms generally appear cream colored or are naturally pigmented green or brown.
The main ESBL-producing bacteria include: Escherichia coli, Klebsiella pneumoniae, Klebsiella oxytoca and Proteus mirabilis. Accurate and rapid detection of ESBL-producing bacteria is essential to assist in the selection of appropriate antibiotic therapy, avoid treatment failures in patients, and prevent further transmission of these multi-drug-resistant organisms.4,5
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For In Vitro Diagnostic Use.
1. Paterson, D.L. and R.A. Bonomo. 2005. Clin. Microbiol. Rev. 18:657-686. 2. Clinical and Laboratory Standards Institute (CLSI). 2012. Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria That Grow Aerobically; Approved Standard, 9th ed. M07-A9. CLSI, Wayne, PA. 3. Shaiks,S., Jamale,F., Shakil, S., Rizvi, S.M.D., Kama, M.A 2014 Saudi J Biol Sci. 2015 Jan; 22(1): 90–101. 4. Harris, A.D., J.C. McGregor, J.A. Johnson, S.M. Strauss, A.C. Moore, H.C. Standiford, J.N. Hebden, and J.G. Morris. 2007. Emerg. Infect. Dis. 57:310-315. 5. Thomson. K. S. 2010. Extended-Spectrum-β-Lactamases, AmpC, and Carbapenamase Issues.J.Clin.Microbiol,48:1019-1025