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dNTP Mix (10 mM each), 0.2 mL - FAQs

dNTP Mix (10 mM each), 0.2 mL - FAQs

View additional product information for dNTP Mix (10 mM each) - FAQs (R0191, R0194, R0192, R0193)

2 product FAQs found

What is the concentration of individual dNTPs in the Thermo Scientific dNTP Mix (Cat. No. R0191)?

The Thermo Scientific dNTP Mix (Cat. No. R0191) contains premixed aqueous solutions of dATP, dCTP, dGTP, and dTTP, each at a final concentration of 10 mM.

What concentration of dNTPs is typically used in a PCR amplification?

The four dNTPs (dATP, dCTP, dTTP, dGTP) are used at a final concentration of 200 µM each or a total of 0.8 mM in standard amplifications. For PCR amplifications that require high fidelity, lower dNTP concentrations (on the order of 10-50 µM) work optimally.

Find additional tips, troubleshooting help, and resources within our PCR and cDNA Synthesis Support Center.