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dNTP Mix (10 mM each), 5 x 1 mL - FAQs

dNTP Mix (10 mM each), 5 x 1 mL - FAQs

View additional product information for dNTP Mix (10 mM each) - FAQs (R0191, R0194, R0192, R0193)

1 product FAQs found

What concentration of dNTPs is typically used in a PCR amplification?

The four dNTPs (dATP, dCTP, dTTP, dGTP) are used at a final concentration of 200 µM each or a total of 0.8 mM in standard amplifications. For PCR amplifications that require high fidelity, lower dNTP concentrations (on the order of 10-50 µM) work optimally.

Find additional tips, troubleshooting help, and resources within our PCR and cDNA Synthesis Support Center.