HI Agar

Cultivate and isolate fastidious and nonfastidious microorganisms using Thermo Scientific™ Remel™ Dehydrated Heart Infusion Agar. In the first media meat infusions were included for the cultivation of bacteria¹. Huntoon prepared a medium using fresh beef heart and peptone which became known as Heart Infusion Agar. He demonstrated that without the addition of enrichment such as animal blood, it could be used to support the growth of nutritionally fastidious microorganisms.

• Ready to use - Solid media
• Used for mass cultivation of bacteria required in vaccine preparation and animal blood can also be added to enable determination of hemolytic reactions by organisms^2,3

This medium contains beef heart infusion and casein peptone that supply nitrogenous compounds and amino acids necessary for the growth of nutritionally fastidious bacteria. Sodium chloride provides essential electrolytes and maintains osmotic equilibrium. Yeast extract is a source of energy and to enhance the bacterial growth it supplies B vitamins.