Malonate Broth,Ewing

Differentiate members of Enterobacteriaceae based on sodium malonate utilization using Thermo Scientific™ Remel™ Malonate Broth (Ewing Modification). Malonate Broth is recommended to differentiate certain subgroups of Salmonella from other Enterobacteriaceae¹. Leifson developed a liquid medium to differentiate Escherichia coli from Enterobacter spp². Ewing et al. modified the Leifson's formulation by adding a trace amount of dextrose and yeast extract to stimulate growth of certain organsims that fail to grow and obseved their reaction on malonate^3,4.

Differentiate members of Enterobacteriaceae based on sodium malonate utilization using Malonate Broth (Ewing Modification).

• Recommended to differentiate certain subgroups of Salmonella from other Enterobacteriaceae¹
• Blue color development due to utilization of malonate is easy to interpret
• Ready to use – Convenience of prepared media

An organism that utilizes sodium malonate as a carbon source and ammonia sulfate as a nitrogen source produces an alkaline reaction and organisms which cannot utilize malonate will ferment dextrose, causes the pH indicator to change from green to yellow thus producing an acid reaction.