Middlebrook 7H9 Broth w/Glycerol

Isolate and cultivate Mycobacterium species using Thermo Scientific™ Remel™ Middlebrook 7H9 Broth w/Glycerol. In 1958, Middlebrook and Cohn first described Middlebrook 7H9 Broth for the cultivation of mycobacteria¹. The supplemental nutrients glycerol, oleic acid, albumin, and dextrose were found to enhance the growtth of most mycobacteria^2,3. This medium is used as a basal medium to prepare inocula for biochemical identification tests and antibiotic susceptibility testing procedures^4,5.

Use Middlebrook 7H9 Broth with Glycerol for isolation and cultivation of Mycobacterium species.

• Recommended for identification and susceptibility testing of Mycobacteria, Nocardiae and other aerobic Actinomycetes by CLSI⁵.
• Ready-to-use – Convenience of prepared media.

Middlebrook 7H9 Broth with Glycerol contains inorganic compounds that supply potassium, sulfur, magnesium, and phosphorus necessary for the growth of mycobacteria. Inorganic copper, iron, zinc, and calcium stimulate growth. Sodium citrate converts to citric acid in solution and holds certain inorganic cations. Ammonium sulfate is a source of nitrogen, dextrose and glycerol provide carbon and albumin binds to the free fatty acids which may be toxic to Mycobacterium species. Catalase destroys toxic peroxides and catalyzes the reaction of iron with molecular oxygen. Biotin is required for carboxylation and decarboxylation reactions and pyridoxine is a precursor of enzymatic activity.