Rhodamine 110, bis-(N-CBZ-L-Aspartyl-L-Glutamyl-L-Valyl-L-Aspartic Acid Amide) (Z-DEVD-R110) - bulk packaging

Catalog number: R22120

Molecular Probes™  Related applications: Cell Viability, Proliferation & Function | Protein Assays and Analysis

Error loading your content!

  Catalog number
Select a plan
Unit size
List price ({{currency}}) Your price ({{currency}}) Availability Qty
{{product.sku}} {{product.sku}}
also known as {{product.formattedSku}} 
{{subscriptionDetails.selectedRatePlan.billingPeriod}} {{subscriptionDetails.selectedRatePlan.billingPeriod}} {{product.availability.message}}
Pro add-ons

Your on-site stock

›› {{supplyCenter.scName}}({{scProduct.stockOnHand}} In stock)
›› {{supplyCenter.scName}}(Out of stock)
›› {{supplyCenter.scName}}
This item is not currently available on-site. Depending on your Supply Center settings you may be able to add the item to cart above else use the Order Non-Stocked Items' tab on the Supply Center home page.
Back to top

Description

This Molecular Probes® caspase substrate is a derivative of the fluorophore rhodamine 110 (R110). Peptides are covalently linked to each of the amino groups on R110, which suppresses the fluorescence of the dye. When the peptides are cleaved by the caspase, the substrate is converted first to the fluorescent monoamide and then to R110, with a further increase in fluorescence. The substrate can be used to continuously measure enzyme activity in cell extracts and purified enzyme preparations using a fluorometer or fluorescence microplate reader.

Substrate Specifications:
• Substrate name: Z-DEVD-R110
• Peptide sequence: DEVD
• Useful to measure: Caspase-3, -6, -7, -8, -10
• Ex/Em of R110 product: (498/521 nm)

Properties of the R110 Reaction Product
The R110 cleavage product has spectral properties similar to those of fluorescein, making it compatible with standard fluorescein filter sets. Because of the large extinction coefficient of R110, signal to background ratios are very good. And, unlike fluorescein, the fluorescence intensity of the rhodamine 110 reaction product is constant from pH 3–9. We also offer an R110 reference standard for use in your assays.

We’ll Make a Custom R110 Substrate for You
If you can’t find what you’re looking for in our stocked list, we’ll prepare a custom R110 substrate for you. Our custom services are efficient and confidential, and we stand by the quality of our work. We are ISO 9001:2000 certified.

For more information about these products, read Detecting Peptidases and Proteases—Section 10.4 in the Molecular Probes® Handbook.

For Research Use Only. Not for human or animal therapeutic or diagnostic use.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Substrate: Z-DEVD-R110
Product Size: 20 mg
Target Enzyme: Caspase
Substrate Type: Caspase Substrate
Detection Method: Fluorescent
Cell Permeability: Cell-Permeant
Substrate Properties: Peptide-Based Substrate
For Use With (Equipment): Microplate Reader, Fluorometer
Shipping Condition: Room Temperature

Contents & storage

Store in freezer (-5 to -30°C).

Documents

Manuals & protocols